在携带重组DNA质粒的大肠杆菌中检测人类γ和β珠蛋白等蛋白质。

Detection of proteins like human gamma and beta globins in Escherichia coli carrying recombinant DNA plasmids.

作者信息

Wilson L B, Grenett H E, Doran D M, Garver F A, Wilson J T

出版信息

Proc Natl Acad Sci U S A. 1979 Nov;76(11):5631-5. doi: 10.1073/pnas.76.11.5631.

DOI:10.1073/pnas.76.11.5631

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC411703/

Abstract

Escherichia coli strain chi 1776 carrying recombinant DNA plasmids containing cDNA copies of human beta or gamma globin mRNAs has been shown by radioimmunoassay to synthesize polypeptides antigenically related to the beta and gamma chains of human hemoglobin. The gamma and beta polypeptides have been enriched from lysates on immunoabsorbent columns containing hemoglobin antibodies and shown to specifically inhibit the antigen-antibody binding between 125I-labeled hemoglobin and the homologous antibody but not other hemoglobin-antihemoglobin reactions. Clone JW151, which is known to contain a complete copy of the coding portion of the gamma globin mRNA, has been shown to produce a protein that reacts specifically with antibody to the chain of fetal hemoglobin, hemoglobin Kenya, and hemoglobin Bart's.

摘要

携带含有人β或γ珠蛋白mRNA cDNA拷贝的重组DNA质粒的大肠杆菌菌株chi 1776，经放射免疫测定已表明能合成与人血红蛋白β链和γ链抗原相关的多肽。γ和β多肽已从含有血红蛋白抗体的免疫吸附柱上的裂解物中富集，并显示能特异性抑制125I标记的血红蛋白与同源抗体之间的抗原-抗体结合，但不抑制其他血红蛋白-抗血红蛋白反应。已知克隆JW151含有γ珠蛋白mRNA编码部分的完整拷贝，已表明它能产生一种与胎儿血红蛋白链、肯尼亚血红蛋白和巴氏血红蛋白的抗体发生特异性反应的蛋白质。

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Detection of proteins like human gamma and beta globins in Escherichia coli carrying recombinant DNA plasmids.在携带重组DNA质粒的大肠杆菌中检测人类γ和β珠蛋白等蛋白质。

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本文引用的文献

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The properties and interactions of the isolated alpha- and beta-chains of human haemoglobin. IV. Immunological studies involving antibodies against the isolated chains.

J Mol Biol. 1966 May;17(1):18-28. doi: 10.1016/s0022-2836(66)80091-8.

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Proc Natl Acad Sci U S A. 1976 May;73(5):1471-5. doi: 10.1073/pnas.73.5.1471.

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Detection and quantitation of the fetal hemoglobin variant Hb F-Malta-I in adults.成人中胎儿血红蛋白变体Hb F-马耳他-I的检测与定量分析。

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