School of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia.
School of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia; Institute of Biophysics SB RAS, Federal Research Center "Krasnoyarsk Science Center SB RAS", 660036 Krasnoyarsk, Russia; Institute of Physics SB RAS, Federal Research Center "Krasnoyarsk Science Center SB RAS", 660036 Krasnoyarsk, Russia.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Jan 15;325:125078. doi: 10.1016/j.saa.2024.125078. Epub 2024 Sep 1.
Luminous marine bacteria are traditionally used as a bioassay due to the convenience and high rate of registering the intensity of their physiological function - luminescence. This study aimed to develop the application of Photobacterium phosphoreum in traditional and novel fields - toxicity monitoring and biotechnology. We demonstrated (1) effects of selenite ions on bioluminescence, and (2) biotransformation of selenite to selenium(0) in the form of nanoparticles. The effects of selenite (SeO) on the intensity of bacterial bioluminescence were studied, and its dependencies on exposure time and concentration of NaSeO were analyzed. Bioluminescence activation and inhibition were revealed; dose-effect dependencies corresponded to the hormesis model. The toxicity of SeO was characterized by an effective concentration of 10 M. Effects of SeO on reactive oxygen species (ROS) in bacterial suspensions were studied. High positive correlations were found between the bioluminescence intensity and ROS content, which indicates the decisive role of ROS and associated redox processes in the bioeffects of selenite ions. Scanning and transmission electron microscopy revealed the presence of nano-structures in the bacteria exposed to selenite. The energy dispersion spectrum detected a high content of selenium in the nanoparticles. The particle size distribution depended on NaSeO concentration; maxima of the distribution varied within 45-55 nm.
发光海洋细菌传统上被用作生物测定，因为它们的生理功能 - 发光的强度很容易且快速地记录下来。本研究旨在将发光细菌（Photobacterium phosphoreum）应用于传统和新领域 - 毒性监测和生物技术。我们证明了：（1）亚硒酸盐离子对生物发光的影响，以及（2）亚硒酸盐向纳米硒的生物转化。研究了亚硒酸盐（SeO）对细菌生物发光强度的影响，并分析了其对暴露时间和 NaSeO 浓度的依赖性。揭示了生物发光的激活和抑制；剂量效应关系符合激素模型。SeO 的毒性特征为 10 μM 的有效浓度。研究了 SeO 对细菌悬浮液中活性氧（ROS）的影响。发现生物发光强度与 ROS 含量之间存在高度正相关，这表明 ROS 及其相关的氧化还原过程在亚硒酸盐离子的生物效应中起决定性作用。扫描和透射电子显微镜显示，在暴露于亚硒酸盐的细菌中存在纳米结构。能量色散光谱在纳米颗粒中检测到高含量的硒。颗粒尺寸分布取决于 NaSeO 浓度；分布的最大值在 45-55nm 范围内变化。
