Zhang Jianyu, Li Jinghui, Qi Renli, Li Shipeng, Geng Xin, Shi Hong, Yu Hualin
Second Department of Neurosurgery, Kunming Medical University First Affiliated Hospital, Kunming, Yunnan, China.
State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China.
Heliyon. 2024 Aug 3;10(16):e35770. doi: 10.1016/j.heliyon.2024.e35770. eCollection 2024 Aug 30.
Glioblastoma (GBM) cells have the potential to switch from being "proliferative cells" to peritumoral "invasive cells". Peritumoral GBM cells have highly invasive properties that allow them to survive surgery, leading to recurrence. The mechanisms underlying the manner in which the tumor microenvironment (TME) regulates the invasiveness of GBM remain unclear. Single-cell RNA sequencing analysis revealed heterogeneity in GBM cells, microglia and macrophages. In this study, the Oncostatin M receptor (OSMR) and leukemia inhibitory factor receptor (LIFR) expression indicated higher invasiveness in core GBM cells. Under environmental stress, the expression of OSMR and LIFR were up-regulated with the effect of hypoxic, acidic, and low-glucose conditions . Functional experiments revealed that TME stress significantly influences the proliferation, migration and invasion of GBM cells. The differences in core/peripheral TMEs in GBM affected the invasive properties, indicating the significant role of OSMR expression within the TME in tumor progression and postoperative therapy.
胶质母细胞瘤（GBM）细胞有从“增殖细胞”转变为瘤周“侵袭性细胞”的潜力。瘤周GBM细胞具有高度侵袭性，使其能够在手术后存活，从而导致复发。肿瘤微环境（TME）调节GBM侵袭性的潜在机制仍不清楚。单细胞RNA测序分析揭示了GBM细胞、小胶质细胞和巨噬细胞的异质性。在本研究中，抑瘤素M受体（OSMR）和白血病抑制因子受体（LIFR）的表达表明核心GBM细胞具有更高的侵袭性。在环境应激下，缺氧、酸性和低糖条件可上调OSMR和LIFR的表达。功能实验表明，TME应激显著影响GBM细胞的增殖、迁移和侵袭。GBM核心/外周TME的差异影响侵袭特性，表明TME内OSMR表达在肿瘤进展和术后治疗中具有重要作用。
