Department of Oral & Maxillofacial Pathology and MicrobiologySharad Pawar Dental College & Hospital, Datta Meghe Institute of Higher Education, Wardha, Maharashtra, 442004, India.
F1000Res. 2024 May 31;12:1326. doi: 10.12688/f1000research.134757.2. eCollection 2023.
The commonest type of cancer in the head and neck region is oral squamous cell carcinoma (OSCC) due to its high rates of occurrence and mortality. The early diagnosis of oral cancer gives better prognosis. Brandwein-Gensler criteria predict the early stage of OSCC cases with a high risk of locoregional recurrence.
To correlate Brandwein-Gensler criteria and depth of invasion of OSCC with three-year survival.
In the study, This study will include 80 random histopathologically-diagnosed cases of OSCC. hematoxylin-eosin (HE)-stained section slides will be used to evaluate, Brandwein and Gensler criteria by three histopathologists in a blinded manner. The depth of invasion assessment will be done from the basement-membrane (BM), in regions where the BM has been lost, as well as from an illustrative line connecting the BM from the neighbouring epithelium to the point of deepest tumour invasion in the connective-tissue stroma with the help of a research microscope (Leica-DMLB2) in resected tissue specimens of OSCC cases.
The present study will find the correlation between Brandwein-Gensler criteria and depth of invasion in OSCC in order to evaluate the locoregional recurrence in OSCC cases. In high-risk OSCC cases, there may be an increased depth of invasion in resected tissues.
We hypothesized that the correlation between Brandwein-Gensler criteria and depth of invasion can be used as an independent predictor for locoregional recurrence in OSCC.
头颈部最常见的癌症是口腔鳞状细胞癌（OSCC），因为其发病率和死亡率都很高。早期诊断口腔癌可获得更好的预后。Brandwein-Gensler 标准可预测具有高局部区域复发风险的 OSCC 早期病例。
将 Brandwein-Gensler 标准和 OSCC 的浸润深度与三年生存率相关联。
在这项研究中，我们将纳入 80 例随机组织病理学诊断为 OSCC 的病例。将使用苏木精-伊红（HE）染色切片来评估 Brandwein 和 Gensler 标准，由三名组织病理学家进行盲法评估。浸润深度评估将从基底膜（BM）进行，如果 BM 已经丢失，则从连接 BM 的说明线进行，该线连接相邻上皮的 BM 与在结缔组织基质中肿瘤侵袭最深点，在 OSCC 病例的切除组织标本中借助研究显微镜（Leica-DMLB2）进行。
本研究将发现 Brandwein-Gensler 标准与 OSCC 浸润深度之间的相关性，以便评估 OSCC 病例的局部区域复发。在高危 OSCC 病例中，切除组织中可能存在更深的浸润。
我们假设 Brandwein-Gensler 标准与浸润深度之间的相关性可作为 OSCC 局部区域复发的独立预测因子。
