Department of Oncology, The Affifiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China.
Immun Inflamm Dis. 2024 Sep;12(9):e70003. doi: 10.1002/iid3.70003.
This study systematically evaluated the relationship between tertiary lymphoid structures (TLS) and clinical pathological features as well as immune infiltrating cells in gastrointestinal cancers.
We searched Web of science, Pubmed, Embase, and Cochrane Library for studies that met the requirements as of July 1, 2023, and the odds ratio, the corresponding 95% confidence interval or mean and standard deviation, were included in the analysis.
We eventually included 20 studies, involving a total of 4856 patients. TLS were found to be significantly associated with T stage, N stage, TNM stage, and tumor size. Moreover, patients with positive TLS showed significantly elevated expression of T-cell related markers, including CD3, CD4, CD8, CD45RO; B-cell related markers, such as CD11c and CD20; and dendritic cell-related marker CD103. On the other hand, positive TLS correlated significantly with low expression of FOXP3 and CD68. Additionally, there was a significant positive association between TLS and overall infiltration of tumor-infiltrating lymphocytes.
The presence of TLS is significantly correlated with the infiltration of various immune cells in gastrointestinal cancers. To determine the ideal balance between the presence of mature TLS and appropriate immune cell infiltration, further high-quality and multicenter clinical studies need to be conducted.
本研究系统评估了三级淋巴结构（TLS）与胃肠道癌临床病理特征及免疫浸润细胞的关系。
我们检索了 Web of science、Pubmed、Embase 和 Cochrane Library 截至 2023 年 7 月 1 日符合要求的研究，并对纳入的研究进行分析，包括比值比、相应的 95%置信区间或平均值和标准差。
最终纳入 20 项研究，共 4856 例患者。TLS 与 T 分期、N 分期、TNM 分期和肿瘤大小显著相关。此外，TLS 阳性患者的 T 细胞相关标志物（如 CD3、CD4、CD8、CD45RO）、B 细胞相关标志物（如 CD11c 和 CD20）和树突状细胞相关标志物 CD103 的表达显著升高，而 FOXP3 和 CD68 的表达显著降低。此外，TLS 与肿瘤浸润淋巴细胞的总体浸润呈显著正相关。
TLS 的存在与胃肠道癌中各种免疫细胞的浸润显著相关。为了确定成熟 TLS 与适当免疫细胞浸润之间的理想平衡，需要进一步开展高质量、多中心的临床研究。
