Spitalnik S L, Schwartz J F, Magnani J L, Roberts D D, Spitalnik P F, Civin C I, Ginsburg V
Blood. 1985 Aug;66(2):319-26.
Anti-My-28 is an IgM kappa monoclonal antibody produced by a hybridoma prepared from spleen cells of a mouse immunized with normal human granulocytes. By immunofluorescence it binds to human granulocytes but not to monocytes and lymphocytes. However, after treating cells with neuraminidase, the antibody also binds to lymphocytes and monocytes and to many leukemic cell lines and patient leukemic blast cells. Anti-My-28 binds to several neutral glycolipids and desialylated gangliosides of leukocytes and erythrocytes as detected by radioimmunoassay and immunostaining of thin-layer chromatograms. It recognizes a sugar sequence in lacto-N-neotetraose, Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc. This tetrasaccharide occurs in the glycolipids paragloboside and sialosylparagloboside, and its distal trisaccharide sequence is found in higher glycolipids and in glycoproteins.
抗My-28是一种IgM κ单克隆抗体，由用正常人粒细胞免疫的小鼠脾脏细胞制备的杂交瘤产生。通过免疫荧光，它与人类粒细胞结合，但不与单核细胞和淋巴细胞结合。然而，在用神经氨酸酶处理细胞后，该抗体也与淋巴细胞、单核细胞以及许多白血病细胞系和患者白血病原始细胞结合。通过放射免疫测定和薄层色谱免疫染色检测，抗My-28与白细胞和红细胞的几种中性糖脂和去唾液酸神经节苷脂结合。它识别乳糖-N-新四糖中的糖序列，即Galβ1-4GlcNAcβ1-3Galβ1-4Glc。这种四糖存在于糖脂副球蛋白和唾液酸副球蛋白中，其远端三糖序列存在于更高的糖脂和糖蛋白中。
