Okada Y, Shimoe T, Muguruma M, Usumoto R, Tsuji T, Jinno K, Moriwaki S, Shin S, Hakomori S
Department of Internal Medicine, Okayama University Medical School, Japan.
Am J Pathol. 1988 Feb;130(2):384-92.
Hepatic expression of sialylated difucosyl Lex antigen (SDLex, NeuAc alpha 2-3Gal beta 1-4(Fuc alpha 1-3)GlcNAc beta 1-3Gal beta 1-4(Fuc alpha 1-3)GlcNAc beta 1-) was studied with monoclonal antibody FH6, which defines this structure. Hepatocytes in the severe form of chronic active hepatitis and liver cirrhosis strongly expressed SDLex. The antigen was only weakly and focally detected in chronic persistent hepatitis. The mild form of chronic active hepatitis showed intermediate expression. SDLex expressed along the liver cell membranes displayed a honeycomb pattern when extensively expressed in the severe form of chronic active hepatitis or in liver cirrhosis. Cytoplasmic expression was faint and focal. Preferential tissue distribution was at the periphery of the hepatic lobules where the distruction of the limiting plate was present. The antigen was also expressed in sinusoidal lining cells and polymorphonuclear cells but not in the biliary epithelia. Hepatocytes expressing SDLex did not express related carbohydrate antigens, ie, Type 2 chain N-acetyllactosamine, Lex, and sialylated Lea. On subcellular fractionation, the microsome fraction contained the majority of the antigen activity. SDS-PAGE and Western blot analysis revealed one major SDLex-active glycoprotein with an apparent molecular weight of 110 kilodaltons. This glycoprotein was different from SDLex-active glycoproteins found in the sera of cancer patients. No ganglioside showed FH6 reactivity. These results indicate that liver cells in active inflammatory lesion expressed a novel glycoprotein carrying SDLex antigen in honeycomblike membrane-associated pattern.
用定义该结构的单克隆抗体FH6研究了唾液酸化二岩藻糖基Lex抗原（SDLex，NeuAcα2-3Galβ1-4（Fucα1-3）GlcNAcβ1-3Galβ1-4（Fucα1-3）GlcNAcβ1-）的肝脏表达情况。在慢性活动性肝炎和肝硬化的严重形式中，肝细胞强烈表达SDLex。在慢性持续性肝炎中，仅微弱且局灶性地检测到该抗原。轻度慢性活动性肝炎表现为中等表达。在慢性活动性肝炎严重形式或肝硬化中广泛表达时，沿肝细胞膜表达的SDLex呈现蜂窝状模式。细胞质表达微弱且局灶性。优先的组织分布位于存在界板破坏的肝小叶周边。该抗原也在窦状隙衬里细胞和多形核细胞中表达，但不在胆管上皮中表达。表达SDLex的肝细胞不表达相关的碳水化合物抗原，即2型链N-乙酰乳糖胺、Lex和唾液酸化Lea。在亚细胞分级分离中，微粒体部分含有大部分抗原活性。SDS-PAGE和蛋白质印迹分析显示一种主要的具有SDLex活性的糖蛋白，其表观分子量为110千道尔顿。这种糖蛋白与癌症患者血清中发现的具有SDLex活性的糖蛋白不同。没有神经节苷脂显示出FH6反应性。这些结果表明，活动性炎性病变中的肝细胞以蜂窝状膜相关模式表达一种携带SDLex抗原的新型糖蛋白。
