State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, PR China.
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, PR China.
Biotechnol Adv. 2024 Dec;77:108448. doi: 10.1016/j.biotechadv.2024.108448. Epub 2024 Sep 11.
L-Phenylalanine (L-Phe) is an important aromatic amino acid, which has been widely used in food, health care products, medicine and other fields. Based on the relatively mature microbial biosynthesis process, a variety of L-phenylalanine-derived compounds have attracted more and more attentions owing to their extensively potential applications in the fields of food, medicine, spices, cosmetics, and pesticides. However, the challenge of biosynthesis of L-phenylalanine-derived compounds remains the issue of low production and productivity. With the development of metabolic engineering and synthetic biology, the biosynthesis of L-phenylalanine has reached a high level. Therefore, the synthesis of L-phenylalanine-derived compounds based on high production strains of L-phenylalanine has broad prospects. In addition, some L-phenylalanine-derived compounds are more suitable for efficient synthesis by exogenous addition of precursors due to their longer metabolic pathways and the inhibitory effects of many intermediate products. This review systematically summarized the research progress of L-phenylalanine-derived compounds, including phenylpyruvate derivatives, trans-cinnamic derivatives, p-coumaric acid derivatives and other L-phenylalanine-derived compounds (such as flavonoids). Finally, the main strategies to improve the production of L-phenylalanine-derived compounds were summarized, and the development trends of the synthesis of L-phenylalanine-derived compounds by microbial method were also prospected.
L-苯丙氨酸（L-Phe）是一种重要的芳香族氨基酸，广泛应用于食品、保健品、医药等领域。基于相对成熟的微生物生物合成工艺，由于其在食品、医药、香料、化妆品和农药等领域的广泛潜在应用，各种 L-苯丙氨酸衍生化合物引起了越来越多的关注。然而，L-苯丙氨酸衍生化合物生物合成的挑战仍然是产量和生产力低的问题。随着代谢工程和合成生物学的发展，L-苯丙氨酸的生物合成已经达到了很高的水平。因此，基于 L-苯丙氨酸高产菌株合成 L-苯丙氨酸衍生化合物具有广阔的前景。此外，由于一些 L-苯丙氨酸衍生化合物的代谢途径较长，许多中间产物具有抑制作用，因此通过外源添加前体更适合高效合成。本综述系统总结了 L-苯丙氨酸衍生化合物的研究进展，包括苯丙酮酸衍生物、反式肉桂酸衍生物、对香豆酸衍生物和其他 L-苯丙氨酸衍生化合物（如类黄酮）。最后，总结了提高 L-苯丙氨酸衍生化合物产量的主要策略，并展望了微生物法合成 L-苯丙氨酸衍生化合物的发展趋势。
