Starr Isabelle, Oen Harrison, McCulloch Alyssa, Frenklakh Sergey, Grandfield Ryan, Choe Hana, Cardinal Kristen O'Halloran
From the Biomedical Engineering Department (I.S., H.O., A.M., K.O.C.), Cal Poly, San Luis Obispo, California.
Stryker Neurovascular (S.F., R.G.), Fremont, California.
AJNR Am J Neuroradiol. 2025 Mar 4;46(3):517-522. doi: 10.3174/ajnr.A8495.
Mechanical thrombectomy devices have potential to injure the vessel during treatment of acute ischemic stroke. The goal of the current work was to tailor in vitro endothelialized silicone models for stent retriever assessment and to evaluate endothelial injury following treatment by various stent retriever designs and sizes. Clinically relevant neurovascular geometries were first modeled out of silicone, then sterilized, coated with fibronectin, placed in bioreactors, seeded with human endothelial cells, and cultivated under flow. Several sizes of 2 different commercially available stent retrievers were then deployed in, and retracted through, vessels. Vessels were immediately harvested and stained. Endothelial injury, identified as denudation, was quantified by using ImageJ. Results illustrated that endothelial injury ranged from 16%-18% in wire/microcatheter-only treated vessels, 37%-61% in 1-pass treatments, and 52%-70% in 2-pass treatments. Overall, this work showcases an in vitro approach for early stage assessment of the extent and location of vascular injury following stent retriever retraction.
机械取栓装置在急性缺血性中风治疗过程中有可能损伤血管。当前研究的目的是定制用于评估取栓支架的体外内皮化硅胶模型，并评估不同设计和尺寸的取栓支架治疗后的内皮损伤情况。首先用硅胶制作出与临床相关的神经血管几何模型，然后进行消毒，涂上纤连蛋白，放入生物反应器中，接种人内皮细胞，并在流动状态下培养。接着将2种不同市售取栓支架的几种尺寸型号放入血管并通过血管进行回撤。血管随即被取出并染色。通过ImageJ软件对被认定为剥脱的内皮损伤进行量化。结果表明，仅使用导丝/微导管治疗的血管内皮损伤率为16% - 18%，单次治疗为37% - 61%，两次治疗为52% - 70%。总体而言，这项研究展示了一种体外方法，用于早期评估取栓支架回撤后血管损伤的程度和位置。
