Biomedical Engineering Department, Cal Poly, San Luis Obispo, California, USA.
Research and Development, Stryker Neurovascular Intervention, Fremont, California, USA.
J Neurointerv Surg. 2021 Aug;13(8):727-731. doi: 10.1136/neurintsurg-2020-016859. Epub 2020 Oct 26.
The goal of this work was to endothelialize silicone aneurysm tubes for use as in vitro models for evaluating endothelial cell interactions with neurovascular devices. The first objective was to establish consistent and confluent endothelial cell linings and to evaluate the silicone vessels over time. The second objective was to use these silicone vessels for flow diverter implantation and assessment.
Silicone aneurysm tubes were coated with fibronectin and placed into individual bioreactor systems. Human umbilical vein endothelial cells were deposited within tubes to create silicone vessels, then cultivated on a peristaltic pump and harvested at 2, 5, 7, or 10 days to evaluate the endothelial cell lining. A subset of silicone aneurysm vessels was used for flow diverter implantation, and evaluated for cell coverage over device struts at 3 or 7 days after deployment.
Silicone vessels maintained confluent, PECAM-1 (platelet endothelial cell adhesion molecule 1) positive endothelial cell linings over time. These vessels facilitated and withstood flow diverter implantation, with robust cell linings disclosed after device deployment. Additionally, the endothelial cells responded to implanted devices through coverage of the flow diverter struts with increased cell coverage over the aneurysm seen at 7 days after deployment as compared with 3 days.
Silicone aneurysm models can be endothelialized and successfully maintained in vitro over time. Furthermore, these silicone vessels can be used for flow diverter implantation and assessment.
本研究旨在对硅酮动脉瘤管进行内皮化处理，用作评估内皮细胞与神经血管设备相互作用的体外模型。首要目标是建立一致且连续的内皮细胞衬里，并随时间评估硅酮血管。第二个目标是使用这些硅酮血管进行血流转向装置的植入和评估。
硅酮动脉瘤管用纤维连接蛋白进行涂层，并放入单独的生物反应器系统中。人脐静脉内皮细胞被沉积在管内以创建硅酮血管，然后在蠕动泵上进行培养，并在第 2、5、7 或 10 天收获，以评估内皮细胞衬里。一部分硅酮动脉瘤管用于血流转向装置的植入，并在植入后第 3 或 7 天评估装置支柱上的细胞覆盖情况。
硅酮血管长时间保持连续的、PECAM-1（血小板内皮细胞黏附分子 1）阳性的内皮细胞衬里。这些血管便于并耐受血流转向装置的植入，在装置部署后显示出强健的细胞衬里。此外，内皮细胞通过覆盖血流转向装置的支柱来响应植入的装置，与植入后 3 天相比，在植入后 7 天观察到动脉瘤上的细胞覆盖增加。
硅酮动脉瘤模型可以进行内皮化处理，并在体外长时间内成功维持。此外，这些硅酮血管可用于血流转向装置的植入和评估。
