Liu Xiangjuan, Zhang Jiahong, Zhao Jun, Cheng Yan, Jiang Dandan
Department of Breast Surgery, Yantaishan Hospital, Yantai, China.
Department of First General Surgery, Yantai Penglai People's Hospital, Yantai, China.
Transl Cancer Res. 2024 Aug 31;13(8):4159-4171. doi: 10.21037/tcr-24-409. Epub 2024 Aug 21.
Tetraspanin 1 (TSPAN1) is a newly discovered protein of the tetrameric protein family encoded by the gene localized in the 1p34 chromosome region. TSPAN1 has been shown to be associated with various malignancies. In this study, we aimed to investigate the prognostic significance of TSPAN1 in breast cancer. Estrogen receptor-positive (ER+) breast cancer is the largest breast cancer subgroup, and ER-targeted therapies have significantly prolonged survival and improved symptoms in advanced breast cancer. TSPAN1 overexpression was found to be associated with a poor prognosis in ER+ breast cancer.
We analyzed the expression of TSPAN1 in breast cancer tissues and cell lines using western blotting and quantitative reverse transcription polymerase chain reaction (RT-qPCR).
TSPAN1 expression was higher in breast cancer cells as compared with normal breast tissue. There was a significant association between a high TSPAN1 level and a low survival rate. Inhibition of TSPAN1 significantly reduced the proliferation and invasion of BT474 cells both and . The downregulation of TSPAN1 in breast cancer cells significantly reduced the levels of p-mitogen-activated protein kinase 1 (MEK1) (S298) and p-extracellular signal-regulating kinase (ERK) 1/2.
TSPAN1 modulates downstream extracellular matrix (ECM) receptor signaling cascades and promotes cellular proliferation and invasion in breast cancer. TSPAN1 inhibition may be a potential new treatment strategy for breast cancer.
四跨膜蛋白1（TSPAN1）是一种新发现的四聚体蛋白家族蛋白，由位于1p34染色体区域的基因编码。TSPAN1已被证明与多种恶性肿瘤相关。在本研究中，我们旨在探讨TSPAN1在乳腺癌中的预后意义。雌激素受体阳性（ER+）乳腺癌是最大的乳腺癌亚组，ER靶向治疗显著延长了晚期乳腺癌患者的生存期并改善了症状。研究发现TSPAN1过表达与ER+乳腺癌的不良预后相关。
我们使用蛋白质免疫印迹法和定量逆转录聚合酶链反应（RT-qPCR）分析了乳腺癌组织和细胞系中TSPAN1的表达。
与正常乳腺组织相比，乳腺癌细胞中TSPAN1表达更高。TSPAN1水平高与低生存率之间存在显著关联。抑制TSPAN1可显著降低BT47细胞系在体外和体内的增殖和侵袭能力。乳腺癌细胞中TSPAN1的下调显著降低了磷酸化丝裂原活化蛋白激酶1（MEK1）（S298）和磷酸化细胞外信号调节激酶（ERK）1/2的水平。
TSPAN1调节下游细胞外基质（ECM）受体信号级联反应，促进乳腺癌细胞的增殖和侵袭。抑制TSPAN1可能是一种潜在的乳腺癌新治疗策略。
