Sunanda Yantrapragada Lakshmi, Parvathaneni Krishna Prasad, Raju T B V G, Seshadri Abitha, Dondapati Gowtam Dev
Department of Conservative Dentistry and Endodontics, Sree Sai Dental College and Research Institute, Srikakulam, Andhra Pradesh, India.
J Conserv Dent Endod. 2024 Jul;27(7):743-749. doi: 10.4103/JCDE.JCDE_212_24. Epub 2024 Jul 8.
The objective of this study was to evaluate the effect of blood and artificial salivary contamination of different root-end filling materials on microleakage using a confocal laser scanning microscope and on marginal adaptation using a scanning electron microscope.
Eighty noncarious single-rooted teeth with mature apices were taken. After retro-cavity preparation, they were randomly assigned into two major groups ( = 40). They were contaminated with blood and artificial saliva, respectively. Each major group was divided randomly into four subgroups ( = 10) and filled as follows: Subgroup A, Biodentine; Subgroup B, bioactive bone cement; Subgroup C, Cention N; and Subgroup D, Bio-C Repair. The samples were sectioned transversely at 1 and 2 mm from the root apex and checked under a confocal laser scanning microscope for microleakage and under an scanning electron microscope for marginal adaptation. The average mean values were calculated. Independent samples -tests, paired -tests, and one-way analysis of variance with Tukey's tests were done to analyze the data.
All the tested materials showed marginal gaps and dye leakage. The Bio-C Repair group showed the least mean marginal gap and dye leakage values, followed by bioactive bone cement, Biodentine, and Cention N, respectively, in both blood and artificial saliva contamination. However, the mean marginal gaps and dye leakage between the major groups were statistically insignificant.
In an overall comparison, Bio-C Repair was found to be superior in terms of marginal adaptation and sealing ability under the test conditions.
本研究的目的是使用共聚焦激光扫描显微镜评估不同根充材料的血液和人工唾液污染对微渗漏的影响，并使用扫描电子显微镜评估对边缘适应性的影响。
选取80颗根尖成熟的非龋单根牙。在进行倒凹洞制备后，将它们随机分为两大组（每组 = 40）。分别用血液和人工唾液污染。每个大组随机分为四个亚组（每组 = 10），并按以下方式进行充填：A组，生物活性玻璃离子水门汀；B组，生物活性骨水泥；C组，Cention N；D组，Bio-C修复材料。将样本从根尖1毫米和2毫米处横向切片，在共聚焦激光扫描显微镜下检查微渗漏情况，并在扫描电子显微镜下检查边缘适应性。计算平均值。采用独立样本t检验、配对t检验以及带有Tukey检验的单因素方差分析来分析数据。
所有测试材料均显示出边缘间隙和染料渗漏。在血液和人工唾液污染情况下，Bio-C修复材料组的平均边缘间隙和染料渗漏值最小，其次分别是生物活性骨水泥、生物活性玻璃离子水门汀和Cention N。然而，各大组之间的平均边缘间隙和染料渗漏在统计学上无显著差异。
在总体比较中，发现在测试条件下，Bio-C修复材料在边缘适应性和密封能力方面更具优势。
