Shalabi M, Saber S, Elsewify T
Department of Endodontics, Ain Shams University, Cairo, Egypt.
Department of Endodontics, The British University, Cairo, Egypt.
Saudi Dent J. 2020 Dec;32(8):373-381. doi: 10.1016/j.sdentj.2019.11.005. Epub 2019 Nov 21.
To evaluate the influence of blood contamination on the bond strength and apatite forming ability of Biodentine used as root-end filling material.
Eighty (n = 80) extracted single-rooted, sound human maxillary anterior teeth were prepared and obturated. Then, the roots were resected, retrograde cavities were prepared and Biodentine was inserted as the root-end filling material. Teeth were then randomly divided into 2 equal groups (n = 40) according to the setting environment of Biodentine i.e., group A where setting took place in human blood and group B where setting took place in deionized water (control group). Teeth were incubated at 37 °C for 45 min to ensure complete setting. Root discs with the filling material in their core were prepared. Push-out bond strength test was performed using a universal testing machine and failure mode was examined. Both groups were aged in HBSS for 30 days. Apatite nucleation was evaluated at one-day, 7-days, and 30-days interval using SEM for morphological analysis and EDX for elemental analysis. Calculation of the Ca/P ratios was performed in addition to XRD for crystal phase analysis.
Blood contamination (group A) resulted in significant reduction of bond strength values. It also affected the amount of apatite deposition on the material surface and interfacial spaces with higher Ca/P ratios than that of the normal stoichiometric hydroxyapatite.
Blood contamination during setting of Biodentine had a detrimental effect on the bond strength and reduced the nucleation of apatite in comparison to non-contaminated group.
评估血液污染对用作根尖充填材料的生物陶瓷的粘结强度和磷灰石形成能力的影响。
选取80颗拔除的单根、完好的人类上颌前牙进行预备和充填。然后，切除牙根，制备倒凹洞形，并将生物陶瓷作为根尖充填材料插入。接着，根据生物陶瓷的凝固环境将牙齿随机分为两组（n = 40），即A组，在人血中凝固；B组，在去离子水中凝固（对照组）。将牙齿在37℃孵育45分钟以确保完全凝固。制备核心带有充填材料的牙根切片。使用万能试验机进行推出粘结强度测试，并检查失败模式。两组均在HBSS中老化30天。使用扫描电子显微镜（SEM）进行形态分析，使用能谱仪（EDX）进行元素分析，在1天、7天和30天的间隔时间评估磷灰石成核情况。除了进行X射线衍射（XRD）分析晶相外，还计算了Ca/P比值。
血液污染（A组）导致粘结强度值显著降低。它还影响了材料表面和界面空间的磷灰石沉积量，其Ca/P比值高于正常化学计量比的羟基磷灰石。
与未受污染组相比，生物陶瓷凝固过程中的血液污染对粘结强度有不利影响，并减少了磷灰石的成核。
