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一种用于高通量分析对……的体液反应的新型全蛋白质组阵列。

A novel pan-proteome array for high-throughput profiling of the humoral response to .

作者信息

Campo Joseph J, Romeis Emily, Oberai Amit, Pablo Jozelyn V, Hung Christopher, Teng Andy A, Shandling Adam D, Phan Amber, Haynes Austin M, Giacani Lorenzo

机构信息

Antigen Discovery, Inc., Irvine, CA, USA.

Department of Medicine, Division of Allergy and Infectious Diseases, University of Washington, Seattle, WA, USA.

出版信息

iScience. 2024 Jul 31;27(9):110618. doi: 10.1016/j.isci.2024.110618. eCollection 2024 Sep 20.

DOI:10.1016/j.isci.2024.110618
PMID:39262771
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11387709/
Abstract

Given the resurgence of syphilis, research endeavors to improve current assays for serological diagnosis and management of this disease are a priority. A proteome-scale platform for high-throughput profiling of the humoral response to () proteins during infection could identify antigens suitable to ameliorate the performance and capabilities of treponemal tests for syphilis. Additionally, because infection-induced immunity is partially protective, profiling the response to outer membrane proteins (OMPs) could help select vaccine candidates. Therefore, we developed a pan-proteome array (PPA) based on the Nichols and SS14 strain complete proteomes and used it to define the immunoglobulin M (IgM) and IgG humoral response to proteins in sera collected longitudinally from long-term infected rabbits and from rabbits that were infected, treated, and re-infected. We identified antigens that could facilitate early diagnosis and immunity to a core set of OMP that could explain protection upon reinfection.

摘要

鉴于梅毒的再度流行，致力于改进当前梅毒血清学诊断和管理检测方法的研究成为当务之急。一个用于高通量分析感染期间对（）蛋白的体液反应的蛋白质组规模平台，能够识别出适合改善梅毒螺旋体检测性能和能力的抗原。此外，由于感染诱导的免疫具有部分保护性，分析对外膜蛋白（OMPs）的反应有助于筛选疫苗候选物。因此，我们基于Nichols和SS14菌株的完整蛋白质组开发了一种全蛋白质组阵列（PPA），并将其用于确定从长期感染的兔子以及感染、治疗和再感染的兔子纵向采集的血清中对蛋白质的免疫球蛋白M（IgM）和IgG体液反应。我们鉴定出了有助于早期诊断的抗原以及一组核心OMP的免疫反应，这组OMP可以解释再感染时的保护作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1aa7/11387709/b7f9d04fb1c1/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1aa7/11387709/e59cecf030be/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1aa7/11387709/12def6dafe6d/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1aa7/11387709/0cde9b00c796/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1aa7/11387709/b3a86d65c3ff/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1aa7/11387709/eaf20013d6ce/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1aa7/11387709/80425940791a/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1aa7/11387709/b7f9d04fb1c1/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1aa7/11387709/e59cecf030be/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1aa7/11387709/12def6dafe6d/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1aa7/11387709/0cde9b00c796/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1aa7/11387709/b3a86d65c3ff/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1aa7/11387709/eaf20013d6ce/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1aa7/11387709/80425940791a/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1aa7/11387709/b7f9d04fb1c1/gr6.jpg

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