结合短读长和长读长测序揭示了……中的地理结构多样性。（原文结尾处不完整）

Combining short- and long-read sequencing unveils geographically structured diversity in .

作者信息

Hoornstra Dieuwertje, Kuleshov Konstantin V, Fingerle Volker, Hepner Sabrina, Wagemakers Alex, Strube Christina, Castillo-Ramírez Santiago, Bockenstedt Linda K, Telford Sam R, Sprong Hein, Platonov Alexander E, Margos Gabriele, Hovius Joppe W

机构信息

Center for Experimental and Molecular Medicine, Amsterdam University Medical Centers, Location Academic Medical Center, Amsterdam, the Netherlands.

Central Research Institute of Epidemiology, Moscow, Russia.

出版信息

iScience. 2024 Jul 30;27(9):110616. doi: 10.1016/j.isci.2024.110616. eCollection 2024 Sep 20.

DOI:10.1016/j.isci.2024.110616

PMID:39262806

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11388275/

Abstract

is an emerging tick-borne human pathogen in the Northern hemisphere. The aim of the current study was to compare whole genome sequences of isolates from different continents. Using a combination of Illumina and PacBio platforms and a novel genome assembly and plasmid typing pipeline, we reveal that the 21 sequenced isolates and publically available genomes from North America, Asia, and Europe form genetically distinct populations and cluster according to their geographical origin, where distinct species are endemic. We identified 20 linear and 17 circular plasmid types and the presence of specific plasmids for isolates originating from different continents. Linear plasmids lp12, lp23, lp41, and lp72 were core plasmids found in all isolates, with lp41 consistently containing the expression site. Our data provide insights into the genetic basis of vector competence, virulence, and pathogenesis of .

摘要

是北半球一种新出现的蜱传人类病原体。本研究的目的是比较来自不同大陆的分离株的全基因组序列。使用Illumina和PacBio平台的组合以及一种新的基因组组装和质粒分型流程，我们发现来自北美、亚洲和欧洲的21个测序分离株和公开可用的基因组形成了遗传上不同的群体，并根据其地理来源聚类，不同的物种在这些地方是地方病。我们鉴定出20种线性和17种环状质粒类型，以及来自不同大陆的分离株中特定质粒的存在。线性质粒lp12、lp23、lp41和lp72是在所有分离株中发现的核心质粒，lp41始终包含表达位点。我们的数据为[病原体名称]的媒介能力、毒力和发病机制的遗传基础提供了见解。