Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Furong Laboratory, Changsha, 410083, China.
Chem Commun (Camb). 2024 Sep 26;60(78):10966-10969. doi: 10.1039/d4cc03438d.
Bioactive NAD mediated multiple biocatalytic pathways in metabolic networks. Refining the structure of NADH oxidase-like (NOX) mimics to efficiently replenish NAD has been promising but challenging in NAD-dependent dehydrogenase electrochemical cascade biosensing. Herein, we discovered that PtO structures, formed lattice oxygen translocation from WO to Pt NPs at the interface, potentially activate and modulate the NOX-like functionality in Pt@WO nanosheets. Incorporating PtO leads to a more positive valence of Pt species within Pt/PtO@WO, where the PtO species serve as preeminent reaction sites for NADH coordination, activation, and dehydrogenation. Consequently, such nanozymes display enhanced NOX-like activity towards NADH oxidation in comparison to Pt@WO. Ultimately, the 650-Pt/PtO@WO nanozyme is employed in an electrochemical cascade biosensor for β-hydroxybutyrate (HB) detection, achieving a calculated detection limit of 25 μM. This study offers insights into PtO activation in Pt-based NOX mimics and supports the future development of NAD/NADH-dependent electrochemical biosensors.
生物活性 NAD 介导代谢网络中的多种生物催化途径。在 NAD 依赖的脱氢酶电化学级联生物传感中，将 NADH 氧化酶模拟物（NOX）的结构精修以有效地补充 NAD 一直是很有前景但具有挑战性的。在此，我们发现 PtO 结构在界面处从 WO 晶格氧迁移到 Pt NPs，这可能会激活和调节 Pt@WO 纳米片中的 NOX 类似功能。引入 PtO 会导致 Pt/PtO@WO 中 Pt 物种的正价态增加，其中 PtO 物种作为 NADH 配位、活化和脱氢的卓越反应位点。因此，与 Pt@WO 相比，这些纳米酶对 NADH 氧化表现出增强的 NOX 样活性。最终，将 650-Pt/PtO@WO 纳米酶用于 β-羟基丁酸 (HB) 的电化学级联生物传感器检测，计算出的检测限为 25 μM。该研究深入了解了 Pt 基 NOX 模拟物中 PtO 的激活，并支持未来开发 NAD/NADH 依赖的电化学生物传感器。
