Yoda Tsuyoshi
Industrial Research Institute Aomori Prefectural Industrial Technology Research Center, 221-10 Yamaguchi Nogi, Aomori City, Aomori, 030-0142, Japan.
Hachinohe Industrial Research Institute Aomori Prefectural Industrial Technology Research Center, 1-4-43 Kita-inter-kogyodanchi, Hachinohe City, Aomori, 039-2245, Japan.
Anal Methods. 2024 Oct 17;16(40):6845-6855. doi: 10.1039/d4ay01479k.
Procyanidins are bioactive compounds present in fruits like apples. These compounds can be extracted and studied for their functional properties. Within the class of procyanidins, procyanidin B2 (PB2) serves as a standard reference. Sake, a traditional Japanese alcoholic beverage, contains ethyl caproate (EC), which adds economic value to the drink. Analyzing PB2 in apple juice and EC in sake, particularly in simple prepared beverages without the use of organic solvents or in their original state, requires the application of binary liposomes composed of 1,2-dioleoyl--glycero-3-phosphocholine (DOPC) and 1,2-dipalmitoyl--glycero-3-phosphocholine (DPPC). In this study, I investigated the size of liposomes and the ratio of solid ordered (So) to liquid disordered (Ld) liposomes. The results indicated that PB2 increased the size of liposomes, while EC decreased it. Additionally, EC influenced the ratio of So/Ld liposomes. These findings provide a foundational understanding for further research on the biophysical and physiological properties of EC and PB2.
原花青素是存在于苹果等水果中的生物活性化合物。这些化合物可以被提取出来并研究其功能特性。在原花青素类别中,原花青素B2(PB2)作为标准参考物。日本传统酒精饮料清酒含有己酸乙酯(EC),这为该饮品增添了经济价值。分析苹果汁中的PB2和清酒中的EC,特别是在不使用有机溶剂的简单制备饮品中或其原始状态下,需要应用由1,2 - 二油酰 - sn -甘油 - 3 - 磷酸胆碱(DOPC)和1,2 - 二棕榈酰 - sn -甘油 - 3 - 磷酸胆碱(DPPC)组成的二元脂质体。在本研究中,我研究了脂质体的大小以及固体有序(So)与液体无序(Ld)脂质体的比例。结果表明,PB2增加了脂质体的大小,而EC则使其减小。此外,EC影响了So/Ld脂质体的比例。这些发现为进一步研究EC和PB2的生物物理和生理特性提供了基础认识。