评价 6 例腹泻马粪菌移植后微生物群变化。
Evaluation of changes in microbiota after fecal microbiota transplantation in 6 diarrheic horses.
机构信息
Department of Biomedical Sciences, Université de Montréal, Montreal, Quebec (Costa, Di Pietro); Department of Clinical Studies, Universidade Estadual de Londrina, Londrina, Paraná, Brazil (Bessegatto, Pereira, Stievani, Gomes, Lisbôa); Department of Clinical Studies, Universidade Filadelfia, Londrina, Paraná, Brazil (Stievani); Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario (Weese).
出版信息
Can Vet J. 2021 Oct;62(10):1123-1130.
The purpose of this study was to characterize the fecal microbiota of horses with acute and chronic diarrhea before and after fecal microbiota transplantation (FMT). Six client-owned horses with acute and chronic diarrhea received FMT from 2 healthy donor horses. Microbiota analysis using next-generation sequencing was performed on fecal samples collected before and 2 and 7 d after FMT. Signs of diarrhea improved in 4 horses, whereas the remaining 2 horses did not survive. There was a significant difference in the number of bacterial species between donors and recipients ( < 0.05). The Order Lactobacillales and the genera , and were increased in the microbiota of diarrheic horses, and genus increased in healthy donors. The results suggest that FMT from the healthy donors was not effective over a 7-day period as it did not change the fecal microbiota of the diarrheic horses. Further research to improve the efficacy of FMT in horses is needed.
本研究旨在描述急性和慢性腹泻马在粪便微生物群移植（FMT）前后的粪便微生物群特征。6 匹患有急性和慢性腹泻的患马从 2 匹健康供体马接受 FMT。在 FMT 前、后 2 天和 7 天收集粪便样本，进行下一代测序的微生物组分析。4 匹马的腹泻症状有所改善，而其余 2 匹马未存活。供体和受体之间的细菌种类数量存在显著差异（<0.05）。在腹泻马的微生物群中，乳杆菌目和属和属增加，而健康供体中属增加。结果表明，健康供体的 FMT 在 7 天内没有效果，因为它没有改变腹泻马的粪便微生物群。需要进一步研究以提高马 FMT 的疗效。
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