Raja Iruthayapandi Selestin, Kim Chuntae, Kang Moon Sung, Joung Yoon Ki, Lee Jong Hun, Han Dong-Wook
Institute of Nano-Bio Convergence, Pusan National University, Busan, 46241, Republic of Korea.
Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Seoul, 02792, Republic of Korea.
Discov Nano. 2024 Sep 13;19(1):149. doi: 10.1186/s11671-024-04110-9.
Functional nanocomposite-based printable inks impart strength, mechanical stability, and bioactivity to the printed matrix due to the presence of nanomaterials or nanostructures. Carbonaceous nanomaterials are known to improve the electrical conductivity, osteoconductivity, mechanical, and thermal properties of printed materials. In the current work, we have incorporated carbon nanofiber nanoparticles (CNF NPs) into methacrylated gelatin (GelMA) to investigate whether the resulting nanocomposite printable ink constructs (GelMA-CNF NPs) promote cell proliferation. Two kinds of printable constructs, cell-laden bioink and biomaterial ink, were prepared by incorporating various concentrations of CNF NPs (50, 100, and 150 µg/mL). The CNF NPs improved the mechanical strength and dielectric properties of the printed constructs. The in vitro cell line studies using normal human dermal fibroblasts (nHDF) demonstrated that CNF NPs are involved in cell-material interaction without affecting cellular morphology. Though the presence of NPs did not affect cellular viability on the initial days of treatment, it caused cytotoxicity to the cells on days 4 and 7 of the treatment. A significant level of cytotoxicity was observed in the highly CNF-concentrated bioink scaffolds (100 and 150 µg/mL). The unfavorable outcomes of the current work necessitate further study of employing functionalized CNF NPs to achieve enhanced cell proliferation in GelMA-CNF NPs-based bioprinted constructs and advance the application of skin tissue regeneration.
基于功能纳米复合材料的可打印油墨，由于纳米材料或纳米结构的存在，可为打印基质赋予强度、机械稳定性和生物活性。已知碳质纳米材料可改善打印材料的导电性、骨传导性、机械性能和热性能。在当前工作中，我们将碳纳米纤维纳米颗粒（CNF NPs）掺入甲基丙烯酸化明胶（GelMA）中，以研究所得的纳米复合可打印油墨结构（GelMA-CNF NPs）是否能促进细胞增殖。通过掺入不同浓度的CNF NPs（50、100和150 µg/mL）制备了两种可打印结构，即载细胞生物油墨和生物材料油墨。CNF NPs改善了打印结构的机械强度和介电性能。使用正常人皮肤成纤维细胞（nHDF）进行的体外细胞系研究表明，CNF NPs参与细胞与材料的相互作用，而不影响细胞形态。虽然在处理初期NPs的存在不影响细胞活力，但在处理的第4天和第7天对细胞产生了细胞毒性。在高浓度CNF生物油墨支架（100和150 µg/mL）中观察到显著水平的细胞毒性。当前工作的不利结果需要进一步研究采用功能化的CNF NPs，以在基于GelMA-CNF NPs的生物打印结构中实现增强的细胞增殖，并推进皮肤组织再生的应用。
