Goto T, Hsieh D P
J Assoc Off Anal Chem. 1985 May-Jun;68(3):456-8.
A detailed fractionation of radioactivity in the milk of goats administered 14C-aflatoxin B1 at low doses was performed. The milk collected in the first 24 h following dosing contained radioactivity equivalent to 0.45-1.1% of the dose given. The radioactivity in each sample was partitioned into 4 fractions: ether, protein, dichloromethane, and water-alcohol. Over 80% of the radioactivity was detected in the dichloromethane fraction, of which over 95% was attributable to aflatoxin M1. No aflatoxin B1 or other known aflatoxin metabolites were detected in any fraction. The results indicate that the major metabolite of aflatoxin B1 in goat milk is aflatoxin M1 and that other metabolites, including conjugates, are of minor significance.
对低剂量给予14C-黄曲霉毒素B1的山羊乳汁中的放射性进行了详细的分级分离。给药后最初24小时收集的乳汁中所含放射性相当于给予剂量的0.45%-1.1%。每个样品中的放射性被分为4个部分：乙醚、蛋白质、二氯甲烷和水-乙醇。超过80%的放射性在二氯甲烷部分被检测到，其中超过95%归因于黄曲霉毒素M1。在任何部分均未检测到黄曲霉毒素B1或其他已知的黄曲霉毒素代谢产物。结果表明，山羊奶中黄曲霉毒素B1的主要代谢产物是黄曲霉毒素M1，其他代谢产物，包括结合物，意义较小。
