McGrew P B, Barnhart H M, Mertens D R, Wyatt R D
J Dairy Sci. 1982 Jul;65(7):1227-33. doi: 10.3168/jds.S0022-0302(82)82334-5.
Eight Holstein cows were selected randomly to determine effects of phenobarbital on the fate of orally administered aflatoxin B1, milk fatty acid profiles, total milk fat, and milk production. Animals were grouped in pairs for one of four treatments: 1) control, 2) oral dosing with sodium phenobarbital for 5 days, 3) dosing with sodium phenobarbital followed by oral dosing with aflatoxin B1, and 4) oral dosing with aflatoxin B1 for 5 days. Daily composite raw milk samples were taken and assayed for aflatoxin M1 by thin-layer chromatography, and milk fatty acid profiles were measured by gas-liquid chromatography. Milk production for all treatments was less than that of controls. Total milk fat was not affected. Both aflatoxin B1 and phenobarbital affected fatty acid distribution, suppressing the amount of fatty acids carbons 8 through 14 while increasing the amount of 16-carbon fatty acids. No treatment effect was significant for the short chain fatty acids of 4 and 6 carbons and the 18-carbon acids oleic, stearic, and linoleic. Pretreatment with phenobarbital resulted in a significant difference between treated and untreated difference between treated and untreated animals. A greater than 50% reduction in the amount of aflatoxin B1 excreted as M1 in the milk was realized.
随机挑选八头荷斯坦奶牛，以确定苯巴比妥对口服黄曲霉毒素B1的代谢、乳脂肪酸谱、总乳脂肪和产奶量的影响。将动物配对分为四种处理之一：1）对照组，2）口服苯巴比妥钠5天，3）先给予苯巴比妥钠，然后口服黄曲霉毒素B1，4）口服黄曲霉毒素B1 5天。每天采集混合的生乳样品，用薄层色谱法检测黄曲霉毒素M1，并用气液色谱法测量乳脂肪酸谱。所有处理的产奶量均低于对照组。总乳脂肪未受影响。黄曲霉毒素B1和苯巴比妥均影响脂肪酸分布，抑制8至14碳脂肪酸的含量，同时增加16碳脂肪酸的含量。对于4碳和6碳的短链脂肪酸以及18碳的油酸、硬脂酸和亚油酸，各处理间差异不显著。苯巴比妥预处理导致处理组和未处理组动物之间存在显著差异。牛奶中以M1形式排泄的黄曲霉毒素B1量减少了50%以上。
