Xie Dan, An Bangquan, Yang Mingyue, Wang Lei, Guo Min, Luo Heng, Huang Shengwen, Sun Fa
Medical College, Guizhou University, Guiyang, China.
Guizhou Provincial People's Hospital, Guiyang, Guizhou, China.
Front Oncol. 2024 Aug 29;14:1389468. doi: 10.3389/fonc.2024.1389468. eCollection 2024.
Leukemia is a malignant tumor with high heterogeneity and a complex evolutionary process. It is difficult to resolve the heterogeneity and clonal evolution of leukemia cells by applying traditional bulk sequencing techniques, thus preventing a deep understanding of the mechanisms of leukemia development and the identification of potential therapeutic targets. However, with the development and application of single-cell sequencing technology, it is now possible to investigate the gene expression profile, mutations, and epigenetic features of leukemia at the single-cell level, thus providing a new perspective for leukemia research. In this article, we review the recent applications and advances of single-cell sequencing technology in leukemia research, discuss its potential for enhancing our understanding of the mechanisms of leukemia development, discovering therapeutic targets and personalized treatment, and provide reference guidelines for the significance of this technology in clinical research.
白血病是一种具有高度异质性和复杂进化过程的恶性肿瘤。应用传统的批量测序技术难以解析白血病细胞的异质性和克隆进化，从而阻碍了对白血病发生发展机制的深入理解以及潜在治疗靶点的识别。然而，随着单细胞测序技术的发展与应用，现在能够在单细胞水平研究白血病的基因表达谱、突变和表观遗传特征，从而为白血病研究提供了新的视角。在本文中，我们综述了单细胞测序技术在白血病研究中的最新应用和进展，讨论了其在增强我们对白血病发生发展机制的理解、发现治疗靶点和个性化治疗方面的潜力，并为该技术在临床研究中的意义提供参考指南。
