Ghiaee Shamloo Ardalan, Zarrinfar Hossein, Jaafari Mahmoud Reza, Yadegari Mohammad Hossein
Department of Medical Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Iran J Microbiol. 2024 Aug;16(4):560-568. doi: 10.18502/ijm.v16i4.16316.
is the second most common species causing infectious diseases and can lead to biofilm resistance. This study aims to adjust and synthesize a liposomal compound of and evaluate its antifungal properties against isolates.
The liposomal formulation of was optimized through the utilization of transmission electron microscopy (TEM), particle size analysis, zeta potential measurement, and UV-visible spectrophotometry. Furthermore, an MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay was conducted on peripheral blood mononuclear cells (PBMCs). The antifungal efficacy was evaluated in accordance with the M27-A3 guideline.
The minimum inhibitory concentrations (MICs) of oil and the liposomal formulation on isolates ranged from 128 to 8 µg/mL and from 250 to 31.25 µg/mL, respectively. The MIC and MIC values of oil and the liposomal formulation were 125, 187, and 32, 96 µg/mL, respectively. The viability percentage of cells treated with the liposomal formulation and free oil was 91% and 85%, respectively.
The cytotoxicity of free was significantly reduced when using nanoliposomes. The liposomal form of showed greater antifungal properties compared to the free extract against isolates.
是引起传染病的第二常见物种，可导致生物膜耐药性。本研究旨在调整和合成的脂质体化合物，并评估其对分离株的抗真菌特性。
通过透射电子显微镜（TEM）、粒度分析、zeta电位测量和紫外可见分光光度法对的脂质体制剂进行优化。此外，对外周血单核细胞（PBMCs）进行了MTT（3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐）测定。根据M27-A3指南评估抗真菌疗效。
油和脂质体制剂对分离株的最低抑菌浓度（MICs）分别为128至8μg/mL和250至31.25μg/mL。油和脂质体制剂的MIC和MIC值分别为125、187和32、96μg/mL。用脂质体制剂和游离油处理的细胞活力百分比分别为91%和85%。
使用纳米脂质体时，游离的细胞毒性显著降低。与游离提取物相比，脂质体形式的对分离株显示出更强的抗真菌特性。
