Inhibitory effect of oil loaded to liposomal nanocarriers on isolates.

BACKGROUND AND OBJECTIVES

is the second most common species causing infectious diseases and can lead to biofilm resistance. This study aims to adjust and synthesize a liposomal compound of and evaluate its antifungal properties against isolates.

MATERIALS AND METHODS

The liposomal formulation of was optimized through the utilization of transmission electron microscopy (TEM), particle size analysis, zeta potential measurement, and UV-visible spectrophotometry. Furthermore, an MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay was conducted on peripheral blood mononuclear cells (PBMCs). The antifungal efficacy was evaluated in accordance with the M27-A3 guideline.

RESULTS

The minimum inhibitory concentrations (MICs) of oil and the liposomal formulation on isolates ranged from 128 to 8 µg/mL and from 250 to 31.25 µg/mL, respectively. The MIC and MIC values of oil and the liposomal formulation were 125, 187, and 32, 96 µg/mL, respectively. The viability percentage of cells treated with the liposomal formulation and free oil was 91% and 85%, respectively.

CONCLUSION

The cytotoxicity of free was significantly reduced when using nanoliposomes. The liposomal form of showed greater antifungal properties compared to the free extract against isolates.