Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloška 4, 1000 Ljubljana, Slovenia.
Institute of Biostatistics and Medical Informatics, Faculty of Medicine, Vrazov trg 2, 1104 Ljubljana, Slovenia.
Diagn Microbiol Infect Dis. 2020 Jun;97(2):115015. doi: 10.1016/j.diagmicrobio.2020.115015. Epub 2020 Feb 8.
We compared 2 molecular tests for detection of herpes simplex viruses 1 and 2 (HSV-1, HSV-2) and varicella-zoster virus (VZV): real-time polymerase chain reaction (RT-PCR) (Argene, BioMerieux, France) performed on an LC480 platform (Roche Applied Science, Mannheim, Germany) and isothermal amplification using a Solana HSV1 + 2/VZV assay (Quidel Corporation Worldwide Headquarters, San Diego, CA) with helicase-dependent amplification performed by a Solana® instrument. With both methods, HSV-1 was detected in 68/291 (23.4%), HSV-2 in 23/291 (7.9%), and VZV in 48/291 (16.5%) skin lesions. Both methods agreed completely only in detection of HSV-2 (kappa = 1). Concordance between Solana HSV1 + 2/VZV and RT-PCR was 98.3% (kappa = 0.95) for HSV-1 and 99.3% (kappa = 0.98) for VZV. Rapid detection of HSV-1, HSV-2, and VZV using the Solana platform is a useful method for routine diagnostics and for urgent swab samples requiring a short turnaround time.
我们比较了 2 种用于检测单纯疱疹病毒 1 和 2（HSV-1、HSV-2）和水痘带状疱疹病毒（VZV）的分子检测方法：实时聚合酶链反应（RT-PCR）（Argene，BioMerieux，法国）在 LC480 平台（罗氏应用科学，曼海姆，德国）上进行，以及使用 Solana HSV1 + 2/VZV 检测（Quidel 公司全球总部，圣地亚哥，CA）进行等温扩增，使用 Solana®仪器进行解旋酶依赖性扩增。使用这两种方法，在 291 个皮肤损伤样本中，分别有 68/291（23.4%）检测到 HSV-1，23/291（7.9%）检测到 HSV-2，48/291（16.5%）检测到 VZV。只有在检测 HSV-2 时，这两种方法完全一致（kappa 值为 1）。Solana HSV1 + 2/VZV 和 RT-PCR 之间的一致性对于 HSV-1 为 98.3%（kappa 值为 0.95），对于 VZV 为 99.3%（kappa 值为 0.98）。使用 Solana 平台快速检测 HSV-1、HSV-2 和 VZV 是常规诊断和需要快速周转时间的紧急拭子样本的有用方法。
