Bandi Z L, Reynolds E S
J Chromatogr. 1985 Jul 5;329(1):57-63. doi: 10.1016/s0021-9673(01)81895-1.
To enhance the UV detectability of hydroxy fatty acids, p-nitrobenzyl (PNB) esters of twenty-two different monohydroxy fatty acids of various chain-lengths (C16-C22) and differing positional isomers were formed using O-(p-nitrobenzyl)-N,N-(diisopropyl)-isourea (PNBDI) as alkylating agent. Reversed-phase and adsorption high-performance liquid chromatography (HPLC) of the twenty-two monohydroxy fatty acid PNB esters were studied. The PNB group did not dominate the chromatographic properties of monohydroxy fatty acids and it did not interfere with the HPLC separation of positional isomers. PNBDI was, however, found to be less than ideal for formation of PNB derivatives of monohydroxy fatty acids because UV absorbing contaminants of PNBDI interfered with the HPLC analyses.
为提高羟基脂肪酸的紫外线可检测性，使用O-(对硝基苄基)-N,N-(二异丙基)-异脲(PNBDI)作为烷基化剂，制备了22种不同链长（C16 - C22）且位置异构体不同的单羟基脂肪酸的对硝基苄基(PNB)酯。研究了这22种单羟基脂肪酸PNB酯的反相和吸附高效液相色谱(HPLC)。PNB基团并未主导单羟基脂肪酸的色谱特性，且不干扰位置异构体的HPLC分离。然而，发现PNBDI对于形成单羟基脂肪酸的PNB衍生物并非理想选择，因为PNBDI的紫外线吸收污染物会干扰HPLC分析。
