Hassan-King M, Greenwood B M, Whittle H C
J Clin Microbiol. 1985 Jul;22(1):136-8. doi: 10.1128/jcm.22.1.136-138.1985.
We measured bactericidal antibodies against meningococci by a method which depends on the inhibition of [3H]thymidine uptake by bacteria in the presence of antibody and complement. A significant increase in antibody activity was found in sera from patients who had recovered from group A meningococcal meningitis (mean inhibition, 38.9 +/- 4.4%) compared with antibody activity present in sera from patients in the acute phase (mean inhibition, 7.0 +/- 2.4%) (P less than 0.001). Similarly, a significant increase in inhibitory activity against group C meningococci was observed 2 weeks after immunization with group C polysaccharide. A close correlation was obtained between the results of the thymidine assay and of a standard colony-counting method and hemagglutination assay. The assay is readily applicable to testing large numbers of samples.
我们采用一种方法来检测针对脑膜炎球菌的杀菌抗体，该方法依赖于在抗体和补体存在的情况下，通过抑制细菌对[3H]胸腺嘧啶核苷的摄取来进行。与急性期患者血清中的抗体活性（平均抑制率为7.0 +/- 2.4%）相比，从A群脑膜炎球菌性脑膜炎康复的患者血清中的抗体活性显著增加（平均抑制率为38.9 +/- 4.4%）（P < 0.001）。同样，在用C群多糖免疫后2周，观察到针对C群脑膜炎球菌的抑制活性显著增加。胸腺嘧啶核苷检测结果与标准菌落计数法和血凝试验结果之间存在密切相关性。该检测方法易于应用于检测大量样本。
