Shalaby M R, Aggarwal B B, Rinderknecht E, Svedersky L P, Finkle B S, Palladino M A
J Immunol. 1985 Sep;135(3):2069-73.
Recombinant human interferon-gamma (rHuIFN-gamma) and natural human tumor necrosis factor beta (nHuTNF-beta) (previously called lymphotoxin), purified to homogeneity, were used to assess their effects on certain functions of human polymorphonuclear neutrophils (PMN) in vitro. The treatment of PMN with 100 U of either rHuIFN-gamma or nHuTNF-beta for 20 min significantly increased their ability to phagocytize 1.5-microns latex beads as detected by flow cytometry. Preparations of recombinant human TNF-beta (rHuTNF-beta) showed activities similar to those of its natural counterpart in activating phagocytosis. In addition, a significant enhancement in PMN-mediated antibody-dependent cellular cytotoxicity was observed after treatment for 2 hr with IFN gamma and both TNF-alpha and TNF-beta. The enhancement by treatment with a combination of rHuIFN-gamma and nHuTNF-beta exceeded the enhancement caused by either agent alone. We also show that although lipopolysaccharide (LPS) is a potent stimulator of PMN function, polymyxin B can block LPS-induced but not lymphokine-induced activation. These data demonstrate new activities for both TNF-alpha and TNF-beta in augmenting the phagocytic and cytotoxic activities of PMN.
将重组人干扰素-γ（rHuIFN-γ）和纯化至同质的天然人肿瘤坏死因子β（nHuTNF-β）（以前称为淋巴毒素）用于体外评估它们对人多形核中性粒细胞（PMN）某些功能的影响。用100 U的rHuIFN-γ或nHuTNF-β处理PMN 20分钟，通过流式细胞术检测发现其吞噬1.5微米乳胶珠的能力显著增强。重组人肿瘤坏死因子β（rHuTNF-β）制剂在激活吞噬作用方面表现出与其天然对应物相似的活性。此外，用IFN-γ以及TNF-α和TNF-β处理2小时后，观察到PMN介导的抗体依赖性细胞毒性显著增强。用rHuIFN-γ和nHuTNF-β联合处理的增强作用超过了单独使用任何一种试剂所引起的增强作用。我们还表明，虽然脂多糖（LPS）是PMN功能的有效刺激剂，但多粘菌素B可阻断LPS诱导的激活，但不能阻断淋巴因子诱导的激活。这些数据证明了TNF-α和TNF-β在增强PMN的吞噬和细胞毒性活性方面的新活性。
