Tene Talia, Bellucci Stefano, Pachacama Joseth, Cuenca-Lozano María F, Tubon-Usca Gabriela, Guevara Marco, La Pietra Matteo, Cruz Salazar Yolenny, Scarcello Andrea, Arias Polanco Melvin, Gahramanli Lala Rasim, Vacacela Gomez Cristian, Caputi Lorenzo S
Department of Chemistry, Universidad Técnica Particular de Loja, Loja 110160, Ecuador.
INFN-Laboratori Nazionali di Frascati, Via E. Fermi 54, 00044 Frascati, Italy.
Nanomaterials (Basel). 2024 Sep 7;14(17):1455. doi: 10.3390/nano14171455.
Herein, we introduce a simple precipitation method for preparing graphene oxide-silver nanoparticle (GO/AgNP) composites, utilizing () seed extract as both a reducing and stabilizing agent. Our research combines the sustainable preparation of graphene oxide (GO) with the green synthesis of silver nanoparticles (AgNPs), aiming to explore the potential of the obtained composite as a novel antibacterial material. To establish a benchmark, the synthesis was also performed using sodium citrate, a conventional reducing agent. The resultant GO/AgNP composites were characterized through several analytical techniques, including scanning electron microscopy (SEM), transmission electron microscopy (TEM), atomic force microscopy (AFM), energy dispersive X-ray spectroscopy (EDS), Raman spectroscopy, X-ray diffraction (XRD), infrared (IR) spectroscopy, and ultraviolet-visible (UV-vis) spectroscopy, confirming the successful functionalization of GO with AgNPs. The antibacterial effectiveness of the composites was systematically assessed against () and (), with nanoparticle concentrations spanning from 0 to 250 µg/mL, utilizing mostly disk diffusion and colony-forming unit (CFU) count assays. The AgNPs were characterized by a size range of 15-50 nm. Notably, the GO/AgNP composite prepared using seed extract demonstrated superior antibacterial activity at all tested concentrations, outperforming both pure GO and the GO/AgNP composite prepared with sodium citrate. The most pronounced antibacterial effect was observed at a concentration of 32.0 µg/mL. Therefore, this innovative synthesis approach may offer a valuable contribution to the development of new therapeutic agents to combat bacterial infections, suggesting further exploration into antibacterial coatings or potential drug development.
在此，我们介绍一种简单的沉淀法来制备氧化石墨烯-银纳米颗粒（GO/AgNP）复合材料，利用（）种子提取物作为还原剂和稳定剂。我们的研究将氧化石墨烯（GO）的可持续制备与银纳米颗粒（AgNPs）的绿色合成相结合，旨在探索所得复合材料作为新型抗菌材料的潜力。为了建立一个基准，还使用传统还原剂柠檬酸钠进行了合成。通过几种分析技术对所得的GO/AgNP复合材料进行了表征，包括扫描电子显微镜（SEM）、透射电子显微镜（TEM）、原子力显微镜（AFM）、能量色散X射线光谱（EDS）、拉曼光谱、X射线衍射（XRD）、红外（IR）光谱和紫外-可见（UV-vis）光谱，证实了AgNPs成功地对GO进行了功能化。利用圆盘扩散法和菌落形成单位（CFU）计数法，系统地评估了复合材料对（）和（）的抗菌效果，纳米颗粒浓度范围为0至250μg/mL。AgNPs的尺寸范围为15-50nm。值得注意的是，使用（）种子提取物制备的GO/AgNP复合材料在所有测试浓度下均表现出优异的抗菌活性，优于纯GO和用柠檬酸钠制备的GO/AgNP复合材料。在浓度为32.0μg/mL时观察到最明显的抗菌效果。因此，这种创新的合成方法可能为开发对抗细菌感染的新型治疗剂做出有价值的贡献，这表明可进一步探索抗菌涂层或潜在的药物开发。
