• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

肝片形吸虫 Opisthorchis viverrini 在糖尿病仓鼠中的转录组变化。

Transcriptome changes of liver fluke Opisthorchis viverrini in diabetic hamsters.

机构信息

Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand - Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand.

Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.

出版信息

Parasite. 2024;31:54. doi: 10.1051/parasite/2024056. Epub 2024 Sep 13.

DOI:10.1051/parasite/2024056
PMID:39269256
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11396942/
Abstract

A recent study in hamsters showed that infection with the liver fluke Opisthorchis viverrini in diabetic hosts worsens the severity of hepatobiliary disease. However, the effects of diabetes on the worm's phenotype and gene expression pattern remain unknown. This study investigated the impact of diabetes on the global gene expression and development of O. viverrini in diabetic hamsters. Parasitological parameters were assessed, and mRNA sequencing with bioinformatic analysis was performed. The study revealed that worm establishment rates in diabetic hamsters were directly correlated with fasting plasma glucose levels. Interestingly, worms collected from diabetic hosts exhibited stunted growth and reduced egg production. Transcriptomic analysis revealed significant alterations in gene expression, with 4314 and 567 differentially expressed genes at 21- and 35-days post-infection, respectively. Gene ontology enrichment analysis highlighted changes in biological processes related to stress response, metabolism, and cellular organization. Notably, genes associated with parasite virulence, including granulin, tetraspanins, and thioredoxins, showed significant upregulation in diabetic hosts. These findings demonstrate the profound impact of host diabetic status on O. viverrini development and gene expression, providing insights into the complex interplay between host metabolism and parasite biology, including molecular adaptations of O. viverrini in hosts. This study contributes to our understanding of opisthorchiasis in the context of metabolic disorders and may inform future strategies for disease management in diabetic human populations.

摘要

最近在仓鼠中的一项研究表明，感染肝片形吸虫（Opisthorchis viverrini）会使糖尿病宿主的肝胆疾病严重程度恶化。然而，糖尿病对蠕虫表型和基因表达模式的影响仍不清楚。本研究调查了糖尿病对糖尿病仓鼠中 O. viverrini 的全局基因表达和发育的影响。评估了寄生虫学参数，并进行了带有生物信息学分析的 mRNA 测序。研究表明，糖尿病仓鼠中蠕虫的建立率与空腹血糖水平直接相关。有趣的是，从糖尿病宿主中收集的蠕虫表现出生长迟缓，产卵减少。转录组分析显示基因表达发生了显著变化，分别在感染后 21 天和 35 天有 4314 个和 567 个差异表达基因。基因本体富集分析突出了与应激反应、代谢和细胞组织相关的生物学过程的变化。值得注意的是，与寄生虫毒力相关的基因，包括颗粒素、四跨膜蛋白和硫氧还蛋白，在糖尿病宿主中显著上调。这些发现表明宿主糖尿病状态对 O. viverrini 发育和基因表达有深远影响，深入了解了宿主代谢与寄生虫生物学之间的复杂相互作用，包括 O. viverrini 在宿主中的分子适应。本研究有助于我们了解代谢紊乱背景下的肝片形吸虫病，并可能为糖尿病人群的疾病管理提供未来策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b5d/11396942/eefab93125a7/parasite-31-54-fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b5d/11396942/4056606348e9/parasite-31-54-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b5d/11396942/623beab90a84/parasite-31-54-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b5d/11396942/be4c141761bd/parasite-31-54-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b5d/11396942/1e1c8726ee37/parasite-31-54-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b5d/11396942/d3e869070521/parasite-31-54-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b5d/11396942/6af7f7a1c446/parasite-31-54-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b5d/11396942/d96f9c026b55/parasite-31-54-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b5d/11396942/cec8f3e3bc2b/parasite-31-54-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b5d/11396942/eefab93125a7/parasite-31-54-fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b5d/11396942/4056606348e9/parasite-31-54-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b5d/11396942/623beab90a84/parasite-31-54-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b5d/11396942/be4c141761bd/parasite-31-54-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b5d/11396942/1e1c8726ee37/parasite-31-54-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b5d/11396942/d3e869070521/parasite-31-54-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b5d/11396942/6af7f7a1c446/parasite-31-54-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b5d/11396942/d96f9c026b55/parasite-31-54-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b5d/11396942/cec8f3e3bc2b/parasite-31-54-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3b5d/11396942/eefab93125a7/parasite-31-54-fig9.jpg

相似文献

1
Transcriptome changes of liver fluke Opisthorchis viverrini in diabetic hamsters.肝片形吸虫 Opisthorchis viverrini 在糖尿病仓鼠中的转录组变化。
Parasite. 2024;31:54. doi: 10.1051/parasite/2024056. Epub 2024 Sep 13.
2
Granulin Expression in Hamsters during Opisthorchis viverrini Infection-Induced Cholangiocarcinogenesis.华支睾吸虫感染诱导仓鼠胆管癌发生过程中颗粒蛋白的表达
Asian Pac J Cancer Prev. 2018 Sep 26;19(9):2437-2445. doi: 10.22034/APJCP.2018.19.9.2437.
3
Vaccination of hamsters with Opisthorchis viverrini extracellular vesicles and vesicle-derived recombinant tetraspanins induces antibodies that block vesicle uptake by cholangiocytes and reduce parasite burden after challenge infection.用华支睾吸虫细胞外囊泡和囊泡衍生的重组四跨膜蛋白免疫仓鼠可诱导产生抗体，阻断囊泡被胆管细胞摄取，并在 challenged 感染后降低寄生虫负荷。
PLoS Negl Trop Dis. 2019 May 28;13(5):e0007450. doi: 10.1371/journal.pntd.0007450. eCollection 2019 May.
4
Genomics of worms, with an emphasis on Opisthorchis viverrini - opportunities for fundamental discovery and biomedical outcomes.蠕虫基因组学，重点是华支睾吸虫——基础发现和生物医学成果的机遇
Parasitol Int. 2017 Aug;66(4):341-345. doi: 10.1016/j.parint.2016.01.005. Epub 2016 Jan 12.
5
Opisthorchis viverrini: Gene expression profiling of carcinogenic adult liver fluke worms using 5' SAGE.猫后睾吸虫：利用5'端基因表达序列分析技术对致癌性成年肝吸虫进行基因表达谱分析
Exp Parasitol. 2008 Dec;120(4):306-13. doi: 10.1016/j.exppara.2008.08.004. Epub 2008 Aug 24.
6
The carcinogenic liver fluke Opisthorchis viverrini is a reservoir for species of Helicobacter.致癌性肝吸虫华支睾吸虫是幽门螺杆菌物种的宿主。
Asian Pac J Cancer Prev. 2015;16(5):1751-8. doi: 10.7314/apjcp.2015.16.5.1751.
7
Molecular characterization of a tetraspanin from the human liver fluke, Opisthorchis viverrini.人肝胆管华支睾吸虫四跨膜蛋白的分子特征。
PLoS Negl Trop Dis. 2012;6(12):e1939. doi: 10.1371/journal.pntd.0001939. Epub 2012 Dec 6.
8
Decreased risk of cholangiocarcinogenesis following repeated cycles of Opisthorchis viverrini infection-praziquantel treatment: Magnetic Resonance Imaging (MRI) and histopathological study in a hamster model.华支睾吸虫反复感染-吡喹酮治疗后胆管癌发生风险降低：仓鼠模型的磁共振成像（MRI）及组织病理学研究
Parasitol Int. 2017 Aug;66(4):464-470. doi: 10.1016/j.parint.2016.04.012. Epub 2016 Apr 30.
9
Cathepsin F cysteine protease of the human liver fluke, Opisthorchis viverrini.肝片形吸虫（Opisthorchis viverrini）组织蛋白酶 F 半胱氨酸蛋白酶。
PLoS Negl Trop Dis. 2009;3(3):e398. doi: 10.1371/journal.pntd.0000398. Epub 2009 Mar 24.
10
Synergistic effects of cagA+ Helicobacter pylori co-infected with Opisthorchis viverrini on hepatobiliary pathology in hamsters.华支睾吸虫与 CagA+幽门螺杆菌协同感染对仓鼠肝胆病理的影响。
Acta Trop. 2021 Jan;213:105740. doi: 10.1016/j.actatropica.2020.105740. Epub 2020 Nov 4.

本文引用的文献

1
Tetraspanins from the liver fluke Opisthorchis viverrini stimulate cholangiocyte migration and inflammatory cytokine production.肝片形吸虫 Opisthorchis viverrini 的四跨膜蛋白可刺激胆管细胞迁移和炎症细胞因子的产生。
Folia Parasitol (Praha). 2023 Sep 16;70:2023.017. doi: 10.14411/fp.2023.017.
2
Effect of experimental hookworm infection on insulin resistance in people at risk of type 2 diabetes.实验性钩虫感染对 2 型糖尿病高危人群胰岛素抵抗的影响。
Nat Commun. 2023 Jul 26;14(1):4503. doi: 10.1038/s41467-023-40263-4.
3
-Current Understanding of the Neglected Hepatobiliary Parasite.
-对被忽视的肝胆寄生虫的当前认识。
Pathogens. 2023 Jun 2;12(6):795. doi: 10.3390/pathogens12060795.
4
The interaction of Schistosoma mansoni infection with diabetes mellitus and obesity in mice.曼氏血吸虫感染与糖尿病和肥胖症在小鼠中的相互作用。
Sci Rep. 2023 Jun 9;13(1):9417. doi: 10.1038/s41598-023-36112-5.
5
Epigenetic regulation in metabolic diseases: mechanisms and advances in clinical study.代谢性疾病中的表观遗传调控：机制及临床研究进展。
Signal Transduct Target Ther. 2023 Mar 2;8(1):98. doi: 10.1038/s41392-023-01333-7.
6
Streptozotocin-Induced Type 1 and 2 Diabetes Mellitus Mouse Models Show Different Functional, Cellular and Molecular Patterns of Diabetic Cardiomyopathy.链脲佐菌素诱导的 1 型和 2 型糖尿病心肌病小鼠模型表现出不同的糖尿病心肌病的功能、细胞和分子特征。
Int J Mol Sci. 2023 Jan 6;24(2):1132. doi: 10.3390/ijms24021132.
7
Intestinal parasites and diabetes: A systematic review and meta-analysis.肠道寄生虫与糖尿病：一项系统综述和荟萃分析
New Microbes New Infect. 2022 Dec 24;51:101065. doi: 10.1016/j.nmni.2022.101065. eCollection 2023 Jan.
8
Knockout of liver fluke granulin, Ov-grn-1, impedes malignant transformation during chronic infection with Opisthorchis viverrini.肝片形吸虫颗粒蛋白（Ov-grn-1）基因敲除阻碍华支睾吸虫感染慢性期的恶性转化。
PLoS Pathog. 2022 Sep 22;18(9):e1010839. doi: 10.1371/journal.ppat.1010839. eCollection 2022 Sep.
9
Evolutionary Adaptations of Parasitic Flatworms to Different Oxygen Tensions.寄生扁虫对不同氧张力的进化适应性
Antioxidants (Basel). 2022 May 31;11(6):1102. doi: 10.3390/antiox11061102.
10
Comparative transcriptome profiles of Schistosoma japonicum larval stages: Implications for parasite biology and host invasion.日本血吸虫幼虫期比较转录组图谱：对寄生虫生物学和宿主入侵的启示。
PLoS Negl Trop Dis. 2022 Jan 13;16(1):e0009889. doi: 10.1371/journal.pntd.0009889. eCollection 2022 Jan.