不等姐妹染色单体交换。一种影响免疫球蛋白基因排列和表达的机制。
Unequal sister chromatid exchange. A mechanism affecting Ig gene arrangement and expression.
作者信息
Tilley S A, Birshtein B K
出版信息
J Exp Med. 1985 Aug 1;162(2):675-94. doi: 10.1084/jem.162.2.675.
Abstract
Two gamma 2a gene forms in the MPC11 mouse myeloma cell line (gamma 2b, K) have been localized to the expressed H chain chromosome, where they exist in tandem downstream of the expressed gamma 2b gene. The gamma 2a gene duplication has apparently occurred by spontaneous, unequal sister chromatid exchange (SCE) in a B lymphoid precursor of MPC11 or in MPC11 itself. It is especially significant that either of the tandem gamma 2a gene forms may be used in the class switch from gamma 2b to gamma 2a production occurring in the MPC11 line, indicating that heavy chain constant region (CH) gene duplication (or deletion) may be a mechanism normally impinging on CH gene expression. The gamma 2a genes of MPC11 undergo further copy number variation via unequal SCE in variants of this line derived by mutagenesis and selection for altered H chain production. The remarkable frequency with which copy number variation occurs in these cells suggests that unequal SCE may play a physiological role in CH gene arrangement and expression.
摘要
在MPC11小鼠骨髓瘤细胞系中的两种γ2a基因形式（γ2b、K）已定位到表达的重链染色体上，它们串联存在于表达的γ2b基因下游。γ2a基因重复显然是通过MPC11的B淋巴细胞前体或MPC11自身中自发的、不等的姐妹染色单体交换（SCE）发生的。特别重要的是，两种串联的γ2a基因形式中的任何一种都可用于MPC11细胞系中发生的从γ2b产生到γ2a产生的类别转换，这表明重链恒定区（CH）基因重复（或缺失）可能是一种通常影响CH基因表达的机制。MPC11的γ2a基因在通过诱变和选择改变重链产生而衍生的该细胞系变体中，通过不等的SCE进一步发生拷贝数变异。这些细胞中拷贝数变异发生的显著频率表明，不等的SCE可能在CH基因排列和表达中发挥生理作用。
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