一种基于 Ru(bpy)(L)@MOF-5 的智能手机辅助电化学发光生物传感器，用于高灵敏度检测 miRNA-21。
A smartphone-assisted electrochemiluminescent biosensor for highly sensitive detection of miRNA-21 based on Ru(bpy)(L)@MOF-5.
机构信息
Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, 350108, China.
Key Laboratory of Sensing Technology and Biomedical Instruments (Guangdong Province), School of Biomedical Engineering, Sun Yat-Sen University, Guangzhou, 510006, China.
出版信息
Mikrochim Acta. 2024 Sep 13;191(10):596. doi: 10.1007/s00604-024-06675-9.
A smartphone-assisted electrochemiluminescence (ECL) strategy based on Ru(bpy)(L) as chromophores confined with metal - organic frameworks (Ru(bpy)(L)@MOF-5) for the signal-amplified detection of miRNA-21 was developed. We synthesized a derivative of tris(2,2'-bipyridyl)ruthenium(II) complex (Ru(bpy)(L)) with high charges, which can be loaded into the MOF-5 by strong electrostatic interaction to prevent from leakage. In addition, nucleic acid cycle amplification was used to quench the signal of Ru(bpy)(L)@MOF-5 by ferrocene. This method was applied to detect the concentration of miRNA-21 ranging from 1.0 × 10-1.0 × 10 M with a low LOD of 7.2 fM. This work demonstrated the construction of a signal quenching strategy ECL biosensor for miRNA using Ru(bpy)(L)@MOF-5 systems and its application in smartphone-assisted ECL detection.
基于金属-有机框架（Ru(bpy)(L)@MOF-5）限制色原体的智能手机辅助电化学发光（ECL）策略，用于信号放大检测 miRNA-21。我们合成了一种带有高电荷的三联吡啶钌（II）配合物（Ru(bpy)(L)）的衍生物，它可以通过强静电相互作用装入 MOF-5 中以防止泄漏。此外，核酸循环扩增用于通过二茂铁猝灭 Ru(bpy)(L)@MOF-5 的信号。该方法用于检测浓度范围为 1.0×10-1.0×10 M 的 miRNA-21，具有低检出限的 7.2 fM。这项工作展示了使用 Ru(bpy)(L)@MOF-5 系统构建用于 miRNA 的信号猝灭策略 ECL 生物传感器及其在智能手机辅助 ECL 检测中的应用。
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