Medical School, School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 210009, China.
Medical School, School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 210009, China; Center of Clinical Laboratory Medicine, Zhongda Hospital, Southeast University, Nanjing, 210009, China; Jiangsu Provincial Key Laboratory of Critical Care Medicine, Southeast University, Nanjing, 210009, China.
Anal Chim Acta. 2022 May 29;1209:339140. doi: 10.1016/j.aca.2021.339140. Epub 2021 Oct 5.
Electrochemiluminescence (ECL) bioanalysis has become increasingly important in various fields from bioanalysis to clinical diagnosis due to its outstanding merits, including low background signal, high sensitivity, and simple instrumentation. Quantum dots (QDs) are a significant theme in ECL bioanalysis since their excellent optical, electrochemical properties, and ease of functionalization endow them with versatile roles and new mechanisms of signal transduction in ECL. Herein, this review details recent advances of QDs-based ECL bioanalysis by using QDs as ECL emitters, coreactants, or ECL resonance energy transfer donors/acceptors, mainly focused on their optical and electrochemical properties and ECL reaction mechanism. In the end, we will discuss the current limitations and future developments in QDs ECL bioanalysis to address the requirement about selectivity, sensitivity, toxicity, and emerging applications.
电化学发光（ECL）生物分析由于其突出的优点，包括低背景信号、高灵敏度和简单的仪器，在从生物分析到临床诊断的各个领域变得越来越重要。量子点（QD）是 ECL 生物分析中的一个重要主题，因为它们优异的光学、电化学性质和易于功能化，使它们具有多种作用和 ECL 信号转导的新机制。本文详细介绍了近年来基于 QD 的 ECL 生物分析的最新进展，包括将 QD 用作 ECL 发射器、共反应物或 ECL 共振能量转移供体/受体，主要集中在它们的光学和电化学性质以及 ECL 反应机制上。最后，我们将讨论 QD 的 ECL 生物分析中目前的局限性和未来的发展，以满足选择性、灵敏度、毒性和新兴应用的要求。
