Feitosa Catherine Biondo, Dos Santos Gabriel Siqueira, Gaeta Natalia Carrillo, Schiavi Gustavo da Silva, Vasconcelos Carla Gasparotto Chande, Filho Jonas Moraes, Heinemann Marcos Bryan, Cortez Adriana
Curso de Pós-Graduação em Saúde Única, Universidade Santo Amaro, São Paulo 04743-030, Brazil.
Laboratório de Zoonoses Bacterianas, Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo 05508-220, Brazil.
Animals (Basel). 2024 Aug 24;14(17):2463. doi: 10.3390/ani14172463.
Enteropathogenic (EPEC) are pathogens associated with gastrointestinal illnesses. Dogs and cats can harbor EPEC, and antimicrobial resistance may impair necessary treatments. This study characterized strains from dogs and cats, focusing on phylogroup classification, virulence factors, and antimicrobial resistance profiles. Ninety-seven isolates from fecal samples of 31 dogs and 3 cats were obtained from a private diagnostic laboratory in Botucatu, Brazil, from March to October 2021. The antimicrobial susceptibility was assessed using the disk diffusion method. Polymerase chain reaction (PCR) was employed to screen for and genes encoding virulence factors, as well as to classify the isolates into phylogroups. Twenty isolates were positive for intimin encoding gene and, consequently, these isolates were classified as EPEC (20.62%). Notably, 5.1% (5/97) of the isolates exhibited extended-spectrum β-lactamase (ESBL) production and 13.4% (13/97) were identified as multidrug-resistant bacteria. Phylogroups A and B2 were the most prevalent, comprising 29.9% (29/97) and 26.8% (26/97) of the bacterial isolates, respectively. This characterization highlights the prevalence of EPEC in domestic animals, emphasizing the potential risk they pose to public health and highlighting the urgency of responsible antimicrobial use in veterinary practices and the important role of laboratories in the surveillance of pathogenic multidrug-resistant bacteria.
肠致病性大肠杆菌（EPEC）是与胃肠道疾病相关的病原体。狗和猫可携带EPEC，而抗菌药物耐药性可能会影响必要的治疗。本研究对来自狗和猫的菌株进行了特征分析，重点关注菌群分类、毒力因子和抗菌药物耐药性谱。2021年3月至10月，从巴西博图卡图的一家私人诊断实验室获得了31只狗和3只猫粪便样本中的97株分离株。采用纸片扩散法评估抗菌药物敏感性。利用聚合酶链反应（PCR）筛选编码毒力因子的基因以及，同时将分离株分类到不同菌群。20株分离株的紧密黏附素编码基因呈阳性，因此，这些分离株被归类为EPEC（20.62%）。值得注意的是，5.1%（5/97）的分离株表现出超广谱β-内酰胺酶（ESBL）产生，13.4%（13/97）被鉴定为多重耐药菌。A群和B2群是最常见的菌群，分别占细菌分离株的29.9%（29/97）和26.8%（26/97）。这一特征突出了EPEC在家畜中的流行情况，强调了它们对公共卫生构成的潜在风险，并突出了兽医实践中合理使用抗菌药物的紧迫性以及实验室在监测致病性多重耐药菌方面的重要作用。
