GDSL in (LpGDSL) Confers Saline-Alkali Resistance to the Plant by Enhancing the Lignin Content and Balancing the ROS.

In order to explore the response mechanism of () to saline-alkali stress, we successfully cloned (GDSL lipase, Gly-Asp-Ser-Leu) from . The qRT-PCR results indicated that the expression was higher in the leaves of , and the expression of the reached the highest level at 12 h in leaves under 11 mM HO, 200 mM NaCl, 25 mM NaCO, and 20 mM NaHCO. The bacteriophage overexpressing was more tolerant than the control under different NaHCO contents. Overexpressed and wild-type plants were analyzed for phenotype, chlorophyll content, O content, HO content, lignin content, and so on. Overexpressed plants had significantly higher resistance than the wild type and were less susceptible to saline-alkali stress. The yeast two-hybrid and BiFC assays demonstrated the existence of an interaction between LpGDSL and LpBCP. The yeast one-hybrid assay and transcriptional activation assay confirmed that B3 transcription factors could act on promoters. Under saline-alkali stress, will promote the expression of , which will then promotes the accumulation of lignin and the scavenging of reactive oxygen species (ROS) to reduce its damage, thus improving the saline-alkali resistance of the plant.