Overexpression of from confers saline-alkali stress (NaHCO) resistance.

with wide distribution is highly tolerant to salinity. The blue copper protein () gene was cloned from , which has the conserved regions of type I copper protein. Moreover, LpCPC has the closest relation to CPC using DNAMAN software and MEGA7 software. qRT-PCR indicated that expression was higher in root and bulb of , and the expression of the gene increased and reached the highest level at 12 h in bulbs under 20 mM NaHCO. The transgenic yeast was more tolerant compared with the control under NaHCO stress. Compared with the wild type, overexpressing plants indicated a relatively lower degree of wilting. In addition, the chlorophyll content, soluble phenol content, and lignin content of overexpressing lines were higher than that of wild-type, whereas the relative conductivity of overexpressing plants was significantly lower than that of wild-type plants. Expression of essential genes including and in salt stress response pathways are steadily higher in overexpression tobacco than that in wild-types. Transgenic lines had much higher levels of and , which are involved in lignin production, compared with wild-type lines. The yeast two-hybrid technique was applied to screen probable interacting proteins interacting with LpCPC. Eight proteins interacted with LpCPC were screened, and five of which were demonstrated to be associated with plant salinity resistance. Overall, the role of gene LpCPC is mediating molecule responses in increasing saline-alkali stress resistance, indicating that it is an essential gene to enhance salt tolerance in .