Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin, China.
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region & Key Laboratory of Molecular Biology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin, China.
Plant Signal Behav. 2024 Dec 31;19(1):2370724. doi: 10.1080/15592324.2024.2370724. Epub 2024 Jul 14.
DC ( DC) plays an important role in the rational utilization of salinized soil. To explore the molecular mechanism of salt-tolerant , the was cloned. LpMYB4 close relationship with and throughout the phylogenetic tree construction. LpMYB4 protein was found to be localized in the nucleus. Prokaryotic and eukaryotic bacterial solution resistance experiments proved that the exogenous introduction of made the overexpression strains obtain better survival ability under saline-alkaline stress. Compared with wild-type plants, tobacco plants overexpressing had better growth and lower leaf wilting and lodging, the content of chlorophyll was higher, the content of hydrogen peroxide and superoxide anion was lower, the activity of peroxidase and superoxide dismutase was higher and the relative conductivity was lower under saline-alkaline stress. The analysis of seed germination and seedling resistance of transgenic plants under salt stress showed that transgenic seeds were more tolerant to salt stress during germination and growth. Yeast two-hybrid and two-luciferase complementation experiments showed that LpMYB4 interacted with yeast two-hybrid and LpGPX6. The analysis of the role of in improving plant saline-alkali resistance is helpful to the transformation of plant germplasm resources and has great significance for agriculture and sustainable development.
DC（DC）在盐碱地的合理利用中起着重要作用。为了探索耐盐性的分子机制，克隆了。LpMYB4 与和在整个系统发育树构建中具有密切关系。发现 LpMYB4 蛋白定位于细胞核中。原核和真核细菌溶液抗性实验证明，外源导入使过表达菌株在盐碱性胁迫下获得更好的生存能力。与野生型植物相比，过表达的烟草植物在盐碱性胁迫下具有更好的生长和更低的叶片萎蔫和倒伏，叶绿素含量更高，过氧化氢和超氧阴离子含量更低，过氧化物酶和超氧化物歧化酶的活性更高，相对电导率更低。对转基因植物在盐胁迫下种子萌发和幼苗抗性的分析表明，在萌发和生长过程中，转基因种子对盐胁迫的耐受性更强。酵母双杂交和双荧光素酶互补实验表明，LpMYB4 与酵母双杂交和 LpGPX6 相互作用。分析在提高植物耐盐碱能力中的作用有助于转化植物种质资源，对农业和可持续发展具有重要意义。
