Department of Biotechnology and Food Microbiology, Faculty of Biotechnology and Food Science, Wrocław University of Environmental and Life Sciences, 51-630 Wrocław, Poland.
Department of Bioorganic Chemistry, Wroclaw University of Economics and Business, 53-345 Wrocław, Poland.
Int J Mol Sci. 2024 Sep 5;25(17):9616. doi: 10.3390/ijms25179616.
Flax ( L.) is an important crop plant with pharmaceutical significance. It is described in pharmacopoeias (the United States Pharmacopeia and the European Pharmacopoeia), which confirms that it (especially the seeds) is a valuable medicinal product. Similar to flax seeds, which accumulate bioactive compounds, flax in vitro cultures are also a rich source of flavonoids, phenolics, lignans and neolignans. In the present study, flax suspension cultures after treatment of the non-pathogenic strain Fo47 were established and analyzed. The study examined the suitability of Fo47 as an elicitor in flax suspension cultures and provided interesting data on the impact of these endophytic fungi on plant metabolism and physiology. Two flax cultivars (Bukoz and Nike) and two compositions of media for flax callus liquid cultures were tested. Biochemical analysis revealed enhanced levels of secondary metabolites (total flavonoid and total phenolic content) and photosynthetically active pigments in the flax callus cultures after treatment with the non-pathogenic fungal strain Fo47 when compared to control, untreated cultures. In cultures with the selected, optimized conditions, FTIR analysis was performed and revealed changes in the structural properties of cell wall polymers after elicitation of cultures with Fo47. The plant cell wall polymers were more strongly bound, and the crystallinity index (Icr) of cellulose was higher than in control, untreated samples. However, lignin and pectin levels were lower in the flax callus liquid cultures treated with the non-pathogenic strain of Fusarium when compared to the untreated control. The potential application of the non-pathogenic strain of for enhancing the synthesis of desired secondary metabolites in plant tissue cultures is discussed.
亚麻（Linum usitatissimum）是一种具有药用意义的重要作物。它被收录在药典（美国药典和欧洲药典）中，这证实了它（尤其是种子）是一种有价值的药用产品。与积累生物活性化合物的亚麻种子类似，体外培养的亚麻也是类黄酮、酚类、木脂素和新木脂素的丰富来源。在本研究中，建立并分析了经非致病性 Fo47 菌株处理的亚麻悬浮培养物。该研究考察了 Fo47 作为亚麻悬浮培养物诱导剂的适用性，并提供了关于这些内生真菌对植物代谢和生理影响的有趣数据。本研究测试了两种亚麻品种（Bukoz 和 Nike）和两种用于亚麻愈伤组织液体培养的培养基组成。生化分析显示，与对照（未经处理的培养物）相比，经非致病性真菌菌株 Fo47 处理后，亚麻愈伤组织培养物中的次生代谢物（总类黄酮和总酚含量）和光合活性色素水平升高。在选择的优化条件下进行了傅里叶变换红外光谱（FTIR）分析，结果显示在 Fo47 诱导培养物后，细胞壁聚合物的结构特性发生了变化。与对照相比，用 Fo47 处理的培养物中植物细胞壁聚合物的结合力更强，纤维素的结晶度指数（Icr）更高。然而，在用非致病性 Fusarium 菌株处理的亚麻愈伤组织液体培养物中，木质素和果胶的含量比未经处理的对照更低。讨论了非致病性菌株 Fo47 在增强植物组织培养中所需次生代谢物合成方面的潜在应用。
