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鉴定和功能研究基因揭示其在干旱胁迫耐受和性生殖中的潜在作用。

Identification and Functional Exploration of Genes Reveal Their Potential Roles in Drought Stress Tolerance and Sexual Reproduction in L. ssp. .

机构信息

Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.

College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.

出版信息

Int J Mol Sci. 2024 Sep 6;25(17):9643. doi: 10.3390/ijms25179643.

DOI:10.3390/ijms25179643
PMID:39273590
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11395553/
Abstract

Gibberellic acid-stimulated Arabidopsis sequences () are a subset of the gibberellin (GA)-regulated gene family and play crucial roles in various physiological processes. However, the genes in have not yet been documented. In this study, we identified and characterized 16 genes in Chinese cabbage ( L. ssp. ). Analysis of the conserved motifs revealed significant conservation within the activation segment of genes. This gene family contains numerous promoter elements associated with abiotic stress tolerance, including those for abscisic acid (ABA) and methyl jasmonate (MeJA). Expression profiling revealed the presence of these genes in various tissues, including roots, stems, leaves, flowers, siliques, and callus tissues. When plants were exposed to drought stress, the expression of decreased notably in drought-sensitive genotypes compared to their wild-type counterparts, highlighting the potentially crucial role of in drought stress. Additionally, exhibited various functions in sexual reproduction dynamics. The findings contribute to the understanding of the function of and provide valuable insights for further exploration of the gene function of the gene in Chinese cabbage.

摘要

赤霉素诱导的拟南芥序列()是赤霉素(GA)调节基因家族的一个子集,在各种生理过程中发挥着关键作用。然而,在白菜(L. ssp.)中,的基因尚未被记录。在这项研究中,我们鉴定并描述了白菜中的 16 个基因()。保守基序分析表明,在基因的激活片段内存在显著的保守性。这个基因家族包含许多与非生物胁迫耐受性相关的启动子元件,包括脱落酸(ABA)和茉莉酸甲酯(MeJA)。表达谱分析表明这些基因存在于各种组织中,包括根、茎、叶、花、角果和愈伤组织。当植物受到干旱胁迫时,与野生型相比,干旱敏感基因型中基因的表达显著下降,这表明在干旱胁迫下基因的表达具有重要作用。此外,基因在有性生殖动态中表现出多种功能。这些发现有助于理解基因的功能,并为进一步探索白菜中基因的功能提供了有价值的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9888/11395553/a69fdc2c8d25/ijms-25-09643-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9888/11395553/0b047573592c/ijms-25-09643-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9888/11395553/155ee756e173/ijms-25-09643-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9888/11395553/3f9e87af33d9/ijms-25-09643-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9888/11395553/ad9255a696e7/ijms-25-09643-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9888/11395553/56e0f2882516/ijms-25-09643-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9888/11395553/a6884159de61/ijms-25-09643-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9888/11395553/3ae4fbd4d30a/ijms-25-09643-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9888/11395553/5e240ae09c56/ijms-25-09643-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9888/11395553/a69fdc2c8d25/ijms-25-09643-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9888/11395553/0b047573592c/ijms-25-09643-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9888/11395553/155ee756e173/ijms-25-09643-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9888/11395553/3f9e87af33d9/ijms-25-09643-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9888/11395553/ad9255a696e7/ijms-25-09643-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9888/11395553/56e0f2882516/ijms-25-09643-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9888/11395553/a6884159de61/ijms-25-09643-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9888/11395553/3ae4fbd4d30a/ijms-25-09643-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9888/11395553/5e240ae09c56/ijms-25-09643-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9888/11395553/a69fdc2c8d25/ijms-25-09643-g009.jpg

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