Potiris Anastasios, Stavros Sofoklis, Voros Charalampos, Christopoulos Panagiotis, Pouliakis Abraham, Savvidis Michael, Papapanagiotou Angeliki, Karampitsakos Theodoros, Topis Spyridon, Vrantza Tereza, Salvara Maria, Gerede Angeliki, Anysiadou Sophia, Daskalakis Georgios, Drakakis Peter, Domali Ekaterini
Third Department of Obstetrics and Gynecology, University General Hospital "ATTIKON", Medical School, National and Kapodistrian University of Athens, 124 62 Athens, Greece.
First Department of Obstetrics and Gynecology, Alexandra Hospital, Medical School, National and Kapodistrian University of Athens, 115 28 Athens, Greece.
J Clin Med. 2024 Sep 6;13(17):5292. doi: 10.3390/jcm13175292.
: Infertility constitutes a significant challenge for couples around the world. Ovarian dysfunction, a major cause of infertility, can manifest with anovulatory cycles, elevated follicle-stimulating hormone levels, and diminished ovarian reserve markers such as anti-Müllerian hormone (AMH) levels or the Antral Follicle Count (AFC). Blood-derived therapies including platelet-rich plasma (PRP) have been used in fertility treatments in women with low ovarian reserve or premature ovarian insufficiency. This prospective clinical cohort study aims to assess the effects of intraovarian PRP therapy on ovarian function in women diagnosed with anovulatory cycles. : The preliminary findings of this prospective cohort study are based on the first 32 patients enrolled. In this study, patients over 40 years old with anovulatory infertility were included. Venous blood samples were collected from each participant for the preparation of autologous platelet-rich plasma (PRP). Each participant received two courses of intraovarian PRP injections using a transvaginal ultrasound-guided approach. Serum levels of reproductive hormones before and after PRP intervention were measured. : This study's results demonstrate a significant improvement in ovarian physiology following transvaginal ultrasound-guided PRP infusion. A 75% increase in Antral Follicle Count (AFC) was observed, which was statistically significant. Furthermore, statistically significant reductions in follicle-stimulating hormone (FSH), luteinizing hormone (LH), and prolactin levels were observed. Serum Vitamin D 1-25 levels were substantially increased after the injection. : These findings highlight the beneficial impact of intraovarian PRP injection in optimizing ovarian function and other metabolic parameters. However, the published literature on this subject is limited and further clinical studies should be conducted to confirm the role of intraovarian PRP in fertility treatments.
不孕不育对全球夫妇来说是一项重大挑战。卵巢功能障碍是不孕不育的主要原因之一，可能表现为无排卵周期、促卵泡生成素水平升高以及卵巢储备标志物减少，如抗苗勒管激素（AMH）水平或窦卵泡计数（AFC）降低。包括富血小板血浆（PRP）在内的血液衍生疗法已被用于治疗卵巢储备功能低下或卵巢早衰的女性不孕症。这项前瞻性临床队列研究旨在评估经阴道超声引导下向卵巢内注射PRP疗法对诊断为无排卵周期的女性卵巢功能的影响。
这项前瞻性队列研究的初步结果基于首批入组的32例患者。在本研究中，纳入了年龄超过40岁的无排卵性不孕症患者。从每位参与者采集静脉血样本以制备自体富血小板血浆（PRP）。每位参与者采用经阴道超声引导的方法接受两个疗程的卵巢内PRP注射。测量PRP干预前后血清生殖激素水平。
本研究结果表明，经阴道超声引导下向卵巢内注入PRP后，卵巢生理功能有显著改善。观察到窦卵泡计数（AFC）增加了75%，具有统计学意义。此外，促卵泡生成素（FSH）、促黄体生成素（LH）和催乳素水平在统计学上有显著降低。注射后血清维生素D 1-25水平大幅升高。
这些发现凸显了卵巢内注射PRP对优化卵巢功能和其他代谢参数的有益影响。然而，关于这一主题的已发表文献有限，应开展进一步的临床研究以证实卵巢内PRP在生育治疗中的作用。
