Key Laboratory of Ministry of Education of Exercise and Physical Fitness, Beijing Sport University, Beijing 100084, China.
Physical Education Department, Xiamen University, Xiamen 361005, China.
Molecules. 2024 Aug 30;29(17):4128. doi: 10.3390/molecules29174128.
To improve exercise performance, the supplement of nutrients has become a common practice before prolonged exercise. Trimethylamine N-oxide (TMAO) has been shown to ameliorate oxidative stress damage, which may be beneficial in improving exercise capacity. Here, we assessed the effects of TMAO on mice with exhaustive swimming, analyzed the metabolic changes, and identified significantly altered metabolic pathways of skeletal muscle using a nuclear magnetic resonance-based (NMR-based) metabolomics approach to uncover the effects of TMAO improving exercise performance of mice. We found that TMAO pre-administration markedly prolonged the exhaustive time in mice. Further investigation showed that TMAO pre-administration increased levels of 3-hydroxybutyrate, isocitrate, anserine, TMA, taurine, glycine, and glutathione and disturbed the three metabolic pathways related to oxidative stress and protein synthesis in skeletal muscle. Our results provide a metabolic mechanistic understanding of the effects of TMAO supplements on the exercise performance of skeletal muscle in mice. This work may be beneficial in exploring the potential of TMAO to be applied in nutritional supplementation to improve exercise performance. This work will lay a scientific foundation and be beneficial to exploring the potential of TMAO to apply in nutritional supplementation.
为了提高运动表现，在长时间运动前补充营养已成为一种常见做法。三甲基胺 N-氧化物（TMAO）已被证明可以改善氧化应激损伤，这可能有助于提高运动能力。在这里，我们评估了 TMAO 对力竭游泳小鼠的影响，分析了代谢变化，并使用基于核磁共振的（NMR-based）代谢组学方法鉴定骨骼肌中显著改变的代谢途径，以揭示 TMAO 提高小鼠运动能力的作用。我们发现 TMAO 预先给药可显著延长小鼠的力竭时间。进一步的研究表明，TMAO 预先给药增加了 3-羟基丁酸、异柠檬酸、鹅肌肽、TMA、牛磺酸、甘氨酸和谷胱甘肽的水平，并扰乱了骨骼肌中与氧化应激和蛋白质合成相关的三条代谢途径。我们的结果提供了 TMAO 补充剂对小鼠骨骼肌运动表现影响的代谢机制理解。这项工作可能有助于探索 TMAO 作为营养补充剂提高运动表现的潜力。这项工作将为探索 TMAO 在营养补充中的应用潜力奠定科学基础，并带来益处。
