Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of Korea.
College of Pharmacy, Korea University, Sejong 30019, Republic of Korea.
Molecules. 2024 Sep 4;29(17):4182. doi: 10.3390/molecules29174182.
As cellular senescence, reactive oxygen species (ROS) accumulate excessively, causing cellular damage. Flavonoids derived from natural products are known for their antioxidant effects and their ability to delay cellular senescence. Previous studies have attempted to mitigate cellular senescence using flavonoids from natural sources. However, the detailed mechanisms and regulatory targets of some flavonoids exhibiting antioxidant effects have not been fully elucidated. Therefore, we screened a library of flavonoids for antioxidant properties. Isoschaftoside, a glycosidic flavonoid, significantly reduced ROS levels in senescent cells. It was found that mitochondrial function was restored, and dependence on glycolysis was reduced in senescent cells treated with isoschaftoside. Additionally, we identified that isoschaftoside suppresses ROS by reducing the expression of and in senescent cells. Taken together, this study establishes a novel mechanism for ROS inhibition and the regulation of cellular senescence by isoschaftoside. Our findings contribute important insights to antioxidant and anti-senescence research.
随着细胞衰老，活性氧（ROS）过度积累，导致细胞损伤。从天然产物中提取的类黄酮以其抗氧化作用和延缓细胞衰老的能力而闻名。先前的研究试图使用天然来源的类黄酮来减轻细胞衰老。然而，一些具有抗氧化作用的类黄酮的详细机制和调节靶点尚未完全阐明。因此，我们筛选了类黄酮库以寻找具有抗氧化特性的化合物。异牡荆苷是一种糖苷类黄酮，可显著降低衰老细胞中的 ROS 水平。研究发现，异牡荆苷处理的衰老细胞中线粒体功能得到恢复，对糖酵解的依赖性降低。此外，我们发现异牡荆苷通过降低衰老细胞中 和 的表达来抑制 ROS。综上所述，本研究确立了异牡荆苷抑制 ROS 和调节细胞衰老的新机制。我们的研究结果为抗氧化和抗衰老研究提供了重要的见解。
