Hasan Mojeer, Azhar Mohd, Nangia Hina, Bhatt Prakash Chandra, Panda Bibhu Prasad
a Microbial and Pharmaceutical Biotechnology Laboratory , Centre for Advanced Research in Pharmaceutical Science, Faculty of Pharmacy , Jamia Hamdard , New Delhi , India.
Prep Biochem Biotechnol. 2016;46(2):116-22. doi: 10.1080/10826068.2014.995807.
In this study astaxanthin production by Phaffia rhodozyma was enhanced by chemical mutation using ethyl methane sulfonate. The mutant produces a higher amount of astaxanthin than the wild yeast strain. In comparison to supercritical fluid technique, high-pressure homogenization is better for extracting astaxanthin from yeast cells. Ultrasonication of dimethyl sulfoxide, hexane, and acetone-treated cells yielded less astaxanthin than β-glucanase enzyme-treated cells. The combination of ultrasonication with β-glucanase enzyme is found to be the most efficient method of extraction among all the tested physical and chemical extraction methods. It gives a maximum yield of 435.71 ± 6.55 µg free astaxanthin per gram of yeast cell mass.
在本研究中，利用甲磺酸乙酯进行化学诱变提高了红发夫酵母虾青素的产量。该突变体产生的虾青素量高于野生酵母菌株。与超临界流体技术相比，高压均质化更适合从酵母细胞中提取虾青素。对用二甲亚砜、己烷和丙酮处理的细胞进行超声处理，其虾青素产量低于用β-葡聚糖酶处理的细胞。在所有测试的物理和化学提取方法中，超声处理与β-葡聚糖酶的组合是最有效的提取方法。每克酵母细胞质量可获得最高产量为435.71±6.55μg的游离虾青素。
