Toman M, Psikal I
Vet Med (Praha). 1985 Jul;30(7):393-400.
The 2-hydroxyethylmethacrylate-particle (HEMA) phagocytosis method in whole blood was adapted to the evaluation of the phagocytic activity of the leucocytes of calves. Several test variants were compared and the best procedure was determined, based on the following principles: keeping the heparin concentration at 10 i. u. in 1 ml of blood at the maximum, using fresh samples, incubating at 37 degrees C for 0.5 to 1 hour, thoroughly agitating the sample after incubation, with possible addition of EDTA. The phagocytic activity of nine calves, 2.5 to 3 months old, was examined by the proposed procedure and 18.6% phagocytic leucocytes were found, the phagocytic index being 7.07%.
将全血中甲基丙烯酸2-羟乙酯颗粒（HEMA）吞噬法应用于犊牛白细胞吞噬活性的评估。比较了几种测试变体，并根据以下原则确定了最佳程序：将肝素浓度保持在每1毫升血液中最高10国际单位，使用新鲜样本，在37℃孵育0.5至1小时，孵育后充分搅拌样本，并可能添加乙二胺四乙酸（EDTA）。采用所提出的程序对9头2.5至3月龄犊牛的吞噬活性进行了检测，发现吞噬白细胞的比例为18.6%，吞噬指数为7.07%。
