Huang Ni, Yang Yi-Na, Huang Jia, Shao Hui-Yan, Li Yan-Lang, Qin Shi-Hui, Li Han-Fen, Shen Xiao-Jiang, Yang Liu, Hu Jiang-Miao
Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Nat Prod Bioprospect. 2024 Sep 14;14(1):52. doi: 10.1007/s13659-024-00476-6.
Dictyophora rubrovalvata is a valuable fungus homologous to food and medicine, and its polysaccharide have been gaining increasing attention because of its plentiful activity. However, the structure and activity of its homogeneous polysaccharide have not been studied enough. In this study, two polysaccharides DRP-I and DRP-II were purified from D. rubrovalvata. Their structures were characterized by chemical composition, monosaccharide composition analysis, methylation analysis and nuclear magnetic resonance spectroscopy. The results showed that DRP-I and DRP-II were neutral heteropolysaccharides with molecular weights of 5.79 × 10 and 1.25 × 10 Da, respectively, which were composed of mannose, galactose, glucose, xylose and fucose. The main chains were → 6)-α-D-Galp-(1 → 6)-α-D-Galp-(2,1 → 6)-α-D-Manp-(2,1 → 6)-α-D-Galp-(1, and branch chains were β-D-Xylp-(1 → 3)-α-L-Fucp-(1 → 4)-α-D-Manp-(1 → and α-D-Galp-(1 → 3)-α-D-Galp-(1 → . The in vitro immunoactivity assays on dendritic cells showed that DRP-I and DRP-II could up-regulate the expression of IL-10 and IL-6 and inhibit the expression of TNF-α in a concentration-dependent manner. This research indicated that DRP-I and DRP-II possessed immunoactivity by balancing the excessive inflammation, and molecular weight is an important factor affecting immunoactivity.
红托竹荪是一种珍贵的药食同源真菌，其多糖因其丰富的活性而受到越来越多的关注。然而，其均一多糖的结构和活性尚未得到充分研究。在本研究中，从红托竹荪中纯化出两种多糖DRP-I和DRP-II。通过化学成分分析、单糖组成分析、甲基化分析和核磁共振光谱对其结构进行了表征。结果表明，DRP-I和DRP-II是中性杂多糖，分子量分别为5.79×10和1.25×10 Da，由甘露糖、半乳糖、葡萄糖、木糖和岩藻糖组成。主链为→6)-α-D-半乳糖-(1→6)-α-D-半乳糖-(2,1→6)-α-D-甘露糖-(2,1→6)-α-D-半乳糖-(1，支链为β-D-木糖-(1→3)-α-L-岩藻糖-(1→4)-α-D-甘露糖-(1→和α-D-半乳糖-(1→3)-α-D-半乳糖-(1→。对树突状细胞的体外免疫活性测定表明，DRP-I和DRP-II能够以浓度依赖的方式上调IL-10和IL-6的表达，并抑制TNF-α的表达。本研究表明，DRP-I和DRP-II通过平衡过度炎症而具有免疫活性，分子量是影响免疫活性的重要因素。
