Marine Geosystems, GEOMAR Helmholtz Centre for Ocean Research Kiel, Wischhofstraße 1-3, 24148 Kiel, Germany.
Bioinformatics Core, University Medical Center Hamburg-Eppendorf, Martinistrasse 51, 20246 Hamburg, Germany.
ISME J. 2024 Jan 8;18(1). doi: 10.1093/ismejo/wrae173.
Chemolithoautotrophic Hydrogenovibrio are ubiquitous and abundant at hydrothermal vents. They can oxidize sulfur, hydrogen, or iron, but none are known to use all three energy sources. This ability though would be advantageous in vents hallmarked by highly dynamic environmental conditions. We isolated three Hydrogenovibrio strains from vents along the Indian Ridge, which grow on all three electron donors. We present transcriptomic data from strains grown on iron, hydrogen, or thiosulfate with respective oxidation and autotrophic carbon dioxide (CO2) fixation rates, RubisCO activity, SEM, and EDX. Maximum estimates of one strain's oxidation potential were 10, 24, and 952 mmol for iron, hydrogen, and thiosulfate oxidation and 0.3, 1, and 84 mmol CO2 fixation, respectively, per vent per hour indicating their relevance for element cycling in-situ. Several genes were up- or downregulated depending on the inorganic electron donor provided. Although no known genes of iron-oxidation were detected, upregulated transcripts suggested iron-acquisition and so far unknown iron-oxidation-pathways.
化能自养氢单胞菌在热液喷口无处不在且丰富。它们可以氧化硫、氢或铁，但没有一种已知的同时利用这三种能源。然而，这种能力在以环境条件高度动态为特征的喷口会很有优势。我们从沿印度洋脊的喷口分离到三种化能自养氢单胞菌菌株，它们可以在三种电子供体上生长。我们展示了在铁、氢或硫代硫酸盐上生长的菌株的转录组数据，分别具有相应的氧化和自养二氧化碳（CO2）固定率、RubisCO 活性、SEM 和 EDX。一株菌的氧化电位的最大估计值分别为 10、24 和 952 mmol 用于铁、氢和硫代硫酸盐氧化，以及 0.3、1 和 84 mmol CO2 固定，每小时每喷口，表明它们对原位元素循环的重要性。根据提供的无机电子供体，有几个基因被上调或下调。尽管没有检测到已知的铁氧化基因，但上调的转录本表明存在铁获取和迄今为止未知的铁氧化途径。
