Ye Jing, Zheng Linjing, Pan Weipeng, Huang Yayan, Zhang Na, Yang Dongda, Yang Yucheng, Zheng Bingde, Zhang Xueqin, Xiao Meitian
College of Chemical Enginering, Huaqiao University, Xiamen 362021, China; Engineering Technology Research Center for Comprehensive Utilization of Marine Biological Resources in Xiamen, Huaqiao University, Xiamen 361021, China.
College of Mechanical Engineering and Automation, Huaqiao University, Xiamen, Fujian 361021, China.
Int J Biol Macromol. 2024 Sep 12;280(Pt 1):135500. doi: 10.1016/j.ijbiomac.2024.135500.
Polysaccharides from sea cucumbers are known for their biological activities, but little is known about those from sea cucumber viscera. The present study isolated a sulfated polysaccharide (SCVP-2) from the viscera of Apostichopus japonicas, which had a molecular weight of 209.1 kDa. SCVP-2 comprised 66.3 % total sugars, 2.1 % uronic acid, 4.5 % proteins, and 25.5 % sulfate groups, containing glucosamine, galactosamine, glucose, galactose, and fucose. FT-IR and NMR analyses identified SCVP-2 as a fucoidan sulfate with sulfation patterns of the fucose branches as Fuc2S, Fuc4S, and Fuc0S. SEM and AFM analyses showed irregular clusters and linear conformations. SCVP-2 demonstrated strong anti-inflammatory properties both in vitro and in vivo. In lipopolysaccharide (LPS)-induced inflammation in macrophage RAW264.7 cells, SCVP-2 significantly reduced nitric oxide (NO) and cytokine secretion (IL-1β, IL-6, TNF-α). Additionally, it downregulated the expression of these cytokine genes. Furthermore, the anti-inflammatory mechanism of SCVP-2 was related to the inhibition of the MAPKs and NF-κB pathways. SCVP-2's anti-inflammatory capacity was confirmed in acute inflammation models, including xylene-induced ear swelling and acetic acid-induced peritoneal capillary permeability, and in high-fat diet-induced systemic low-grade chronic inflammation. In conclusion, SCVP-2 exhibits significant anti-inflammatory activity, suggesting its potential for development as a functional food ingredient or therapeutic agent for inflammation-related diseases.
海参多糖以其生物活性而闻名，但对海参内脏中的多糖却知之甚少。本研究从仿刺参内脏中分离出一种硫酸化多糖（SCVP - 2），其分子量为209.1 kDa。SCVP - 2含有66.3%的总糖、2.1%的糖醛酸、4.5%的蛋白质和25.5%的硫酸基团，包含氨基葡萄糖、半乳糖胺、葡萄糖、半乳糖和岩藻糖。傅里叶变换红外光谱（FT - IR）和核磁共振（NMR）分析确定SCVP - 2为一种岩藻聚糖硫酸酯，岩藻糖分支的硫酸化模式为Fuc2S、Fuc4S和Fuc0S。扫描电子显微镜（SEM）和原子力显微镜（AFM）分析显示其为不规则簇状和线性构象。SCVP - 2在体外和体内均表现出强大的抗炎特性。在脂多糖（LPS）诱导的巨噬细胞RAW264.7细胞炎症中，SCVP - 2显著降低了一氧化氮（NO）和细胞因子分泌（IL - 1β、IL - 6、TNF - α）。此外，它下调了这些细胞因子基因的表达。此外，SCVP - 2的抗炎机制与丝裂原活化蛋白激酶（MAPKs）和核因子κB（NF - κB）信号通路的抑制有关。SCVP - 2的抗炎能力在急性炎症模型中得到证实，包括二甲苯诱导的耳肿胀和醋酸诱导的腹腔毛细血管通透性增加，以及高脂饮食诱导的全身性低度慢性炎症。总之，SCVP - 2具有显著的抗炎活性，表明其有潜力开发成为功能性食品成分或用于治疗炎症相关疾病的治疗剂。
