College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China; Key Laboratory of Water and Sediment Sciences, Ministry of Education of China, Beijing 100871, China.
College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China; State Environmental Protection Key Laboratory of All Material Fluxes in River Ecosystems, Beijing 100871, China.
Sci Total Environ. 2024 Dec 1;954:176239. doi: 10.1016/j.scitotenv.2024.176239. Epub 2024 Sep 12.
Bacterial communication could affect their interactions, but whether this regulation has "intelligence" is still unknown. Here, we operated an anammox reactor under temperature gradient from 35 °C to 15 °C. As results, expression abundance of bacterial communication genes increased by 12 % significantly after temperature declined. Division of labor among distinct signal molecules was evidenced by complementary roles of acyl-homoserine lactones (AHLs) and diffusible signal factor (DSF) in affecting bacterial interactions and niche differentiation respectively. DSF based inter-and intra-communication helped bacteria match their investments and rewards during cross-feedings. When temperature was below 25 °C, transcription regulator Clp governed by DSF inclined to promote folate and molybdenum cofactor biosynthesis, which coincidentally benefited one anammox species more than another. Meanwhile, for the anammox species with lower benefits, Clp also inclined to decrease biosynthesis of costly tryptophan and vitamin B1 rewarding others. Interestingly, bacterial communication inclined to influence the bacteria with many cooperators in the community or with high capacity to export cofactors for cross-feedings when temperature decreased. As results, these bacteria were enriched which could lead to closer interactions in whole community to adapt to low temperatures. The discovered intelligence of bacterial communication opened another window for understanding bacterial sociobiology.
细菌之间的交流可能会影响它们的相互作用，但这种调节是否具有“智能”尚不清楚。在这里，我们在温度梯度从 35°C 到 15°C 的条件下运行了一个厌氧氨氧化反应器。结果表明，温度下降后，细菌交流基因的表达丰度显著增加了 12%。不同信号分子之间的分工通过酰高丝氨酸内酯 (AHLs) 和可扩散信号因子 (DSF) 分别在影响细菌相互作用和生态位分化方面的互补作用得到了证明。基于 DSF 的种间和种内交流有助于细菌在交叉喂养过程中匹配它们的投资和回报。当温度低于 25°C 时，由 DSF 调控的转录调节因子 Clp 倾向于促进叶酸和钼辅酶的生物合成，这恰好使一种厌氧氨氧化菌比另一种受益更多。同时，对于受益较少的厌氧氨氧化菌，Clp 也倾向于减少昂贵的色氨酸和维生素 B1 的生物合成，以回报其他细菌。有趣的是，当温度下降时，细菌交流倾向于影响群落中合作者较多或具有较强因子外排能力进行交叉喂养的细菌。结果，这些细菌得到了富集，这可能导致整个群落中的相互作用更加紧密，以适应低温。细菌交流的这种“智能性”为理解细菌社会生物学开辟了另一个视角。
