间充质干细胞条件培养基用于受损肌肉相关生物标志物的新见解：大鼠模型的体内研究
New Insight in Using of Mesenchyme Stem Cell Conditioning Medium for the Impaired Muscle related Biomarkers: In vivo Study with Rat Model.
作者信息
Kartika Ronald Winardi, Sidharta Veronika Maria, Djuartina Tena, Sartika Cynthia Retna, Timotius Kris Herawan
机构信息
Department of Surgery, Faculty of Medicine and Health Sciences, Krida Wacana Christian University, West Jakarta, Indonesia.
Master of Biomedical, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, North Jakarta, Indonesia.
出版信息
Ann Afr Med. 2024 Oct 1;23(4):674-679. doi: 10.4103/aam.aam_205_23. Epub 2024 Sep 14.
AIMS AND OBJECTIVES
This study aimed to investigate the effects of Umbilical Cord Mesencymal Stem Cell Conditioning Medium (UC MSC-CM) administration on body weight recovery and the level of four molecular biomarkers, namely Superoxide Dismutase (SOD), vascular Endothelial Growth Factor (VEGF), C-Reactive Protein (CRP), and myostatin.
MATERIALS AND METHODS
Secretome was injected intramuscularly twice at 1.5 mL (day 7 and 14) into the right thigh of high-dose, short-term galactose-induced aging rats. The data of day 7 (before) and day 21 (after the administration) were evaluated. The body weights and the four biomarkers were measured before (day 7) and after intervention (day 21).
RESULTS
This study showed that the UC MSC-CM intramuscular administrations did not influence body weight regeneration. However, it could increase SOD and VEGF levels and decrease CRP and myostatin levels.
CONCLUSION
Treatment with UC MSC-CM is a promising and potential agent in treating sarcopenia.
目的
本研究旨在探讨脐带间充质干细胞条件培养基（UC MSC-CM）给药对体重恢复以及四种分子生物标志物水平的影响，这四种生物标志物分别为超氧化物歧化酶（SOD）、血管内皮生长因子（VEGF）、C反应蛋白（CRP）和肌肉生长抑制素。
材料与方法
将分泌组以1.5毫升的剂量分两次（第7天和第14天）肌肉注射到高剂量、短期半乳糖诱导衰老大鼠的右大腿。评估第7天（给药前）和第21天（给药后）的数据。在干预前（第7天）和干预后（第21天）测量体重和四种生物标志物。
结果
本研究表明，肌肉注射UC MSC-CM对体重恢复没有影响。然而，它可以提高SOD和VEGF水平，降低CRP和肌肉生长抑制素水平。
结论
UC MSC-CM治疗是一种有前景且有潜力的治疗肌肉减少症的药物。
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