Department of Ophthalmology, The Second Clinical Medical College, Chongqing Medical University, Chongqing, China.
Department of Refractive Center, Chongqing Nanping Aire Eye Hospital, China.
Immunopharmacol Immunotoxicol. 2024 Dec;46(6):773-784. doi: 10.1080/08923973.2024.2402365. Epub 2024 Sep 15.
Ocular chemical burns are a leading cause of blindness. The cornea is injured by alkali-induced oxidative disturbances and an inflammatory response. The aim of this study was to evaluate the protective effects of aloin, an antioxidant, and anti-inflammatory compound, on corneal alkali burn.
Mice eyes were injured by NaOH and subsequently treated with aloin eye drop and intraperitoneal injection. Pathological characteristics of the eyes were examined, and corneal samples were collected for further analysis.
Aloin diminished neutrophil infiltration and the production of proinflammatory cytokines. Aloin also attenuated apoptosis in human corneal epithelial cells (HCEs) by reducing oxidative stress through the activation of the Nrf2 pathway. Additionally, aloin suppressed the formation of neutrophil extracellular traps (NETs) and inhibited their deposition on the cornea. Moreover, aloin mitigated alkali-induced apoptosis in HCEs caused by NETs.
These findings suggest that aloin has potential as an antioxidant and anti-inflammatory compound for treating corneal alkali burn by inhibiting NETs formation and promoting Nrf2.
眼部化学烧伤是失明的主要原因。角膜因碱诱导的氧化紊乱和炎症反应而受损。本研究旨在评估抗氧化、抗炎化合物芦荟素对角膜碱烧伤的保护作用。
通过 NaOH 损伤小鼠眼睛，随后用芦荟素滴眼和腹腔注射进行治疗。检查眼睛的病理特征，并收集角膜样本进行进一步分析。
芦荟素减少了中性粒细胞浸润和促炎细胞因子的产生。芦荟素还通过激活 Nrf2 通路减轻氧化应激，从而减少人角膜上皮细胞 (HCE) 的凋亡。此外，芦荟素抑制中性粒细胞胞外陷阱 (NETs) 的形成，并抑制其在角膜上的沉积。此外，芦荟素减轻了 NETs 引起的 HCE 碱诱导的细胞凋亡。
这些发现表明，芦荟素具有通过抑制 NETs 的形成和促进 Nrf2 作为治疗角膜碱烧伤的抗氧化和抗炎化合物的潜力。
