C2VN, Aix-Marseille Univ, INRAE, INSERM, 27, boulevard Jean Moulin, 13385 Marseille Cedex 5, France.
Department of Science and Technology - Food and Nutrition Research Institute, Bicutan, Taguig City, Philippines.
Food Funct. 2024 Sep 30;15(19):9995-10006. doi: 10.1039/d4fo03087g.
Lutein (L) and zeaxanthin (Z) are involved in visual function and could prevent age-related macular degeneration and chronic diseases and improve cognitive performances. Adipose tissue is the main storage site for these xanthophylls (Xanth). The factors affecting their concentrations in this tissue remain poorly understood but in animal models, genetic variations in apolipoprotein E and β-carotene oxygenase 2 have been associated with adipose tissue L concentration. Therefore, the aims of this study were to better characterize the interindividual variability of adipose tissue Xanth concentration and to identify single nucleotide polymorphisms (SNPs) associated with it. Periumbilical subcutaneous adipose tissue samples were collected on 6 occasions in 42 healthy adult males and L and Z concentrations were measured by HPLC. Participants had their whole genome genotyped and the associations of 3589 SNPs in 49 candidate genes with the concentrations of L and Z were measured. Mean L and Z concentrations were 281 ± 27 and 150 ± 14 nmol g proteins, respectively. There was no significant correlation between plasma and adipose tissue Xanth concentrations, although the correlation for L approached significance (Pearson's = 0.276, = 0.077). Following univariate filtering, 109 and 97 SNPs were then entered into a partial least squares regression analysis to identify the combination of SNPs that explained best adipose tissue concentration of L and Z, respectively. A combination of 7 SNPs in , , and , explained 58% of the variability in adipose tissue L concentration while 11 SNPs located in or near , , , , , , explained 53% of the variance in adipose tissue Z concentration. This suggests that some genetic variations influence the concentrations of these Xanth in adipose tissue and could therefore indirectly influence the health effects of these compounds. Clinical Trial Registry: https://ClinicalTrials.gov registration number NCT02100774.
叶黄素 (L) 和玉米黄质 (Z) 参与视觉功能，可预防年龄相关性黄斑变性和慢性病，并改善认知表现。脂肪组织是这些类胡萝卜素 (Xanth) 的主要储存场所。影响其在该组织中浓度的因素仍知之甚少，但在动物模型中，载脂蛋白 E 和 β-胡萝卜素加氧酶 2 的遗传变异与脂肪组织 L 浓度有关。因此，本研究的目的是更好地描述脂肪组织 Xanth 浓度的个体间变异性，并确定与其相关的单核苷酸多态性 (SNP)。在 42 名健康成年男性中，每 6 次采集脐周皮下脂肪组织样本，通过 HPLC 测量 L 和 Z 的浓度。参与者进行了全基因组基因分型，并测量了 49 个候选基因中的 3589 个 SNP 与 L 和 Z 浓度的相关性。L 和 Z 的平均浓度分别为 281 ± 27 和 150 ± 14 nmol g 蛋白。尽管 L 的相关性接近显著（Pearson's = 0.276， = 0.077），但血浆和脂肪组织 Xanth 浓度之间没有显著相关性。经过单变量过滤后，然后将 109 个和 97 个 SNP 分别输入偏最小二乘回归分析，以确定分别解释脂肪组织 L 和 Z 浓度的最佳 SNP 组合。位于 、 、 和 中的 7 个 SNP 的组合解释了脂肪组织 L 浓度变异性的 58%，而位于 或附近的 11 个 SNP 、 、 、 、 、 、 解释了脂肪组织 Z 浓度变异性的 53%。这表明一些遗传变异会影响这些 Xanth 在脂肪组织中的浓度，从而间接影响这些化合物的健康影响。临床试验注册：https://ClinicalTrials.gov 注册号 NCT02100774。
